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| The bidirectional promoter site between the rebG and rebO genes was replaced with a TRP1 bidirectional promoter cassette and the unidirectional promoter in the upstream region of the rebR gene was replaced with a MET15 cassette in which only one promoter was incorporated into the amplicon used for recombination (i.e., aunidirectional promoter cassette) (SI Appendix). |
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ÖÁ×ðľ³æ (ÖøÃûдÊÖ)
- ·ÒëEPI: 204
- Ó¦Öú: 103 (¸ßÖÐÉú)
- ½ð±Ò: 15293.5
- ºì»¨: 41
- Ìû×Ó: 1611
- ÔÚÏß: 431.6Сʱ
- ³æºÅ: 3856202
- ×¢²á: 2015-05-08
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fjtony163: ½ð±Ò+1, ¹ÄÀøÓ¦Öú 2016-03-18 12:58:45
bxzc123(fjtony163´ú·¢): ½ð±Ò+15, ´ú·¢ 2016-04-17 04:13:08
fjtony163: ·ÒëEPI+1, ´ú·¢ 2016-04-17 04:13:14
fjtony163: ½ð±Ò+1, ¹ÄÀøÓ¦Öú 2016-03-18 12:58:45
bxzc123(fjtony163´ú·¢): ½ð±Ò+15, ´ú·¢ 2016-04-17 04:13:08
fjtony163: ·ÒëEPI+1, ´ú·¢ 2016-04-17 04:13:14
| ÒÔTRP1Ë«ÏòÆô¶¯×ÓºÐÌæ»»rebG ºÍ rebO»ùÒòÖ®¼äµÄË«ÏòÆô¶¯×Óλµã£¬²¢ÒÔMET15ºÐÌæ»»rebR»ùÒòÉÏÓÎÇøÓòÖеĵ¥ÏòÆô¶¯×Ó¡£ÔÚMET15ºÐÖÐÖ»ÓÐÒ»¸öÆô¶¯×ÓÒýÈëÓÃÓÚÖØ×éµÄÀ©Ôö×ÓÖУ¨È磬һ¸öµ¥ÏòÆô¶¯×ӺУ©£¨¸½Â¼SI) |
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