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An alternative approach to save markers is to generate synthetic polycistronic genes where all genes in the construct are under the control of a single promoter and where all ORFs are separated by a sequence encoding the viral 2A peptide that results in co-translational cleavage, hence, resulting in the formation of independent enzymes

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loveqiuqiu: ½ð±Ò+10, ·­ÒëEPI+1, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, лл 2016-03-16 19:50:56
An alternative approach to save markers is to generate synthetic polycistronic genes where all genes in the construct are under the control of a single promoter and where all ORFs are separated by a sequence encoding the viral 2A peptide that results in co-translational cleavage, hence, resulting in the formation of independent enzymes

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