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| An alternative approach to save markers is to generate synthetic polycistronic genes where all genes in the construct are under the control of a single promoter and where all ORFs are separated by a sequence encoding the viral 2A peptide that results in co-translational cleavage, hence, resulting in the formation of independent enzymes |
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loveqiuqiu: ½ð±Ò+10, ·ÒëEPI+1, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, лл 2016-03-16 19:50:56
loveqiuqiu: ½ð±Ò+10, ·ÒëEPI+1, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, лл 2016-03-16 19:50:56
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An alternative approach to save markers is to generate synthetic polycistronic genes where all genes in the construct are under the control of a single promoter and where all ORFs are separated by a sequence encoding the viral 2A peptide that results in co-translational cleavage, hence, resulting in the formation of independent enzymes ÁíÒ»¸ö±£´æ±ê¼ÇµÄ·½·¨ÊÇÉú³ÉÈ˹¤ºÏ³É¶à˳·´×Ó»ùÒò£¬ÈÃËùÓеĻùÒò¶¼´¦ÓÚÒ»¸öÆô¶¯×ӵĿØÖÆÖ®Ï£¬²¢ÈÃËùÓеÄORFs¶¼±»Ò»¸ö±àÂ벡¶¾2AËõ°±ËáµÄÐòÁиô¿ª£¬µ¼Ö¹²·ÒëÇи´Ó¶øÐγɸ÷×Ô¶ÀÁ¢µÄø¡£ |
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