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[求助]
注射器发酵装置
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最近在文献中看到了一种简易的BMP测定方法，利用100mL注射器作为发酵装置，想求教使用过这种方法的前辈一些问题： 我看的文献中提到了使了100mL注射器、30mL基质、顶部三通阀、硅胶密封、恒温振荡器， 那么在实际进行中有什么需要注意的地方么（包括实验过程以及发酵装置的细节）？ 对于注射器有什么要求么？ 对于基质（主要是基质的产期能力）有没有限制？ 谢谢大家！  下面是文献中的方法，引用的参考文献都是书、下不到 The anaerobic digestion tests were conducted based on the well-established biochemical methane potential (BMP) procedure, as described in the guideline VDI 4630 (VDI, 2006). In detail, the batch fermentations were carried out in 100 mL glass syringes according to the Hohenheimer Biogas Yield Test (HBT) (Helffrich and Oechsner, 2003). To ensure anaerobic conditions and to collect the biogas, the syringes were gas tightened by using mediumviscosity silicone paste (GE Bayer Silicones GmbH & Co KG, Erkrath, Germany). Three way valves allowed sampling of the biogas. For stirring and heating, the syringes were placed in a continuously rotating vertical disc (5 rpm) located in a 42 C heating chamber. VDI, 2006. Fermentation of Organic Materials – Characterisation of the Substrate, Sampling, Collection of Material Data, Fermentation Tests. Beuth Verlag, Berlin. Helffrich, D., Oechsner, H., 2003. Hohenheimer biogas yield test – comparing various laboratory methods on biomass fermentation. Landtechnik 58, 148– 149. |
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