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Of the 12 bidirectional promoter cassettes we tested, eight led to the production of rebeccamycin (1). Six productive promoter marker gene cassettes, one for each marker gene, were selected for use as promoter replacement tools in this study For each of the six active cassettes, we also con- structed a corresponding set of cassettes with promoters containing 16-bp spacing between the −10 and −35 sequences ， which is reported to generically reduce promoter strength (24). All 12 DNAs were TOPO-cloned to provide are newable source of each cassette for use in promoter exchange experiment 还有张图，求大神帮忙  UZ1M$7JYKD7M(S}KB{ZYTCV.png |
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