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虫号: 2384387
注册: 2013-03-28
专业: 微生物生理与生物化学

[求助] 求翻译。。。

Yeast homologous recombination-based promoter engineering for the activation of silent natural product biosynthetic gene clusters

To solve this problem, we added random 15-bp in- sulator sequences and unique natural Streptomyces RBSs (19) to
24 promoters identified by Seghezzi et al. (18). The spacer and
RBS sequences were matched to give an overall GC content of
～65% for each promoter element. Pairs of promoter/spacer/RBS sequences were synthesized as primers containing 20-bp sequences homologous to the ends of
yeast selectable marker genes.

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