24小时热门版块排行榜

返回列表

本帖产生 1 个翻译EPI ，点击这里进行查看

scauyyg

金虫 (小有名气)

应助: 0 (幼儿园)
金币: 4662.8
散金: 768
红花: 1
帖子: 82
在线: 26.6小时
虫号: 3737683
注册: 2015-03-14
性别: GG
专业: 植物生理与生化

[求助] 帮忙翻译，满意追加金币

For the gene detection about N and Si uptake and assimilation, leaves or roots were collected from different treatments for analysis. For BPH treatment, leaf sheathes were collected for analysis of defense gene expression. There were 3 biological replicates in each treatment. All samples were immediately frozen in liquid nitrogen and stored at -80 ℃, and ground to fine powder in liquid nitrogen just before the total RNA extraction. Total RNA was extracted from 0.1 g leaf sample using TRIzol Reagent (Life Technologies) according to the manufacture’s instructions. First-strand cDNA was synthesized from 1 μg of total RNA using M-MLV Reverse Transcription Kit (Invitrogen) according to the manufacture’s instructions. Real-time PCR was performed using SYBR Premix Ex Taq II (Tli RNaseH Plus, Takara) with 7500 Fast Real-Time PCR Systems (Applied Biosystems). Reaction condition for thermal cycling were 90 ℃ for 30 s, 40 cysles of 95 ℃ for 15 s, 60 ℃ for 30 s, 72 ℃for 30 s (elongation and collected fluorescence data). Melting curve analysis and agarose gel electrophoresis were carried out to verify the amplicon specificity. Transcripts of targeted genes were calculated by the double-standard curves method, and the rice housekeeping gene OsActin was used for sample normalization as an endogenous control. All the specific primers in this study were listed in Table S1. There were three biological replicates for each treatment.

发自小木虫Android客户端

回复此楼

» 猜你喜欢

写了一篇“相变储能技术在冷库中应用”的论文，论文内容以实验为主，投什么期刊合适？已经有3人回复
需要合成515-64-0，50g，能接单的留言已经有3人回复
最近几年招的学生写论文不引自己组发的文章已经有10人回复
中科院杭州医学所招收博士生一名（生物分析化学、药物递送）已经有3人回复
A期刊撤稿已经有5人回复
临港实验室与上科大联培博士招生1名已经有8人回复
26申博自荐已经有7人回复
想换工作。大多数高校都是评职称时认可5年内在原单位取得的成果吗？已经有4人回复
带资进组求博导收留已经有9人回复
求助大佬们，伤口沾上了乙腈已经有6人回复

1楼 2016-03-10 15:13:23

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

络络依然

版主 (知名作家)

翻译EPI: 9
应助: 0 (幼儿园)
贵宾: 21.943
金币: 101714
散金: 7049
红花: 140
沙发: 17
帖子: 5942
在线: 2657.9小时
虫号: 2025515
注册: 2012-09-24
性别: MM
专业: 环境分析化学
管辖: 文献求助

【答案】应助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
爱与雨下: 金币+1, 最好直接paste 2016-03-16 07:29:20
scauyyg(fjtony163代发): 金币+30, 代发 2016-04-11 23:30:42
scauyyg(fjtony163代发): 金币+20, 代发 2016-04-11 23:30:50
fjtony163: 翻译EPI+1, 代发 2016-04-11 23:30:57