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smallcat227木虫 (著名写手)
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[交流]
求助：高温下培养基的保水
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请问各位，在高温下（50度以上）培养微生物时如何防止琼脂培养基失水过快？我们现在是用保鲜膜，不过会影响透气，还有更好的方法么，在培养基中加甘油？ 谢谢各位！ |
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smallcat227
木虫 (著名写手)
- 应助: 1 (幼儿园)
- 金币: 9746
- 散金: 2016
- 帖子: 1238
- 在线: 104.7小时
- 虫号: 502444
- 注册: 2008-02-14
- 专业: 环境微生物学
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这是老外们的回复： "We have to incubate plates for weeks at 37C. To keep them from drying we use shrink seals. They are plastic seals seals that shrink around your plate once exposed to a high heat source for a few seconds. They allow ventilation and plates dont dry out "; "use humid chambers. Take a box, fill it with (autoclaved) water (depends on the contamination risk), add a "second floor" above the water surface (we use glassware as pile, then put plasic as floor on it but you can use whatever you have and is suitable for your experiment). Like little Venice in the lab. You can incubate your samples for a very long time, but you should check time by time to prevent your plates from becoming gondolas!"; "Adding an extra 1-1.5% agar to your medium during preparation may help to reduce water loss. We would do this, and also keep the plates in loosely closed plastic bags" |
9楼2008-10-15 20:05:47
smallcat227
木虫 (著名写手)
- 应助: 1 (幼儿园)
- 金币: 9746
- 散金: 2016
- 帖子: 1238
- 在线: 104.7小时
- 虫号: 502444
- 注册: 2008-02-14
- 专业: 环境微生物学
3楼2008-10-14 20:56:18
4楼2008-10-14 22:09:03
6楼2008-10-14 23:20:31