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jackchen129

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[交流] PCR agarose是non-denaturing吗 已有1人参与

PCR agarose是non-denaturing吗?可以用来验证提取的RNA的完整性吗?

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oliviashane

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Non-denaturing RNA electrophoresis eliminates the need for hazardous chemicals, but due to intramolecular interactions, RNA molecules can form extensive double-stranded structures that are quite difficult to disrupt. As a result, accurate sizing of RNA molecules is not always possible under non-denaturing conditions. Native RNA electrophoresis is therefore typically used to assess the overall quality of total RNA. Denaturing electrophoresis is recommended to precisely determine the size and integrity of RNA molecules.
2楼2016-03-01 11:09:55
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jackchen129

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引用回帖:
2楼: Originally posted by oliviashane at 2016-02-29 22:09:55
Non-denaturing RNA electrophoresis eliminates the need for hazardous chemicals, but due to intramolecular interactions, RNA molecules can form extensive double-stranded structures that are quite diff ...

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3楼2016-03-01 11:24:31
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