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Ìæ±ðÈËÇóÖúÎÄÏ×һƪ£¬¡¶Interactions between arbuscular mycorrhizal fungi (Glomus intraradices,Glomeromycota) and amoebae (Acanthamoeba castellanii,Protozoa) in the rhizosphere of rice (Oryza sativa).¡·

×÷ÕßÊÇHerdler S, Kreuzer K, Scheu S, BonkowskiM (2008)
ÆÚ¿¯ÃûSoil Biol Biochem

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Interactions between arbuscular mycorrhizal fungi (Glomus intraradices, Glomeromycota) and amoebae (Acanthamoeba castellanii, Protozoa) in the rhizosphere of rice (Oryza sativa)

S. Herdlera, K. Kreuzera, S. Scheua and M. Bonkowskia, b, ,

aDepartment of Animal Ecology, Institute for Zoology, Technical University Darmstadt, Schnittspahnstr. 3, D-64287 Darmstadt, Germany bDepartment of Terrestrial Ecology and Rhizosphere Research, Institute for Zoology, University of Cologne, Weyertal 119, D-50931 Köln, Germany

Received 10 July 2007;  revised 26 September 2007;  accepted 28 September 2007.  Available online 22 October 2007.




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Abstract
Rice plants (Oryza sativa L.) were grown in microcosms containing soil with a diverse bacterial community (control) and inoculated either with an axenic arbuscular mycorrhizal fungus (Glomus intraradices) or an axenic inoculum of protozoan grazers of bacteria (Acanthamoeba castellanii), or both, in a factorial design.

Amoebae and mycorrhiza affected the root architecture of rice in opposite directions, with mycorrhiza reducing and protozoa increasing early root growth. Rice biomass did not increase in presence of mycorrhiza (¡Á1.08), but strongly increased in presence of Acanthamoebae (¡Á1.29). The positive effects of amoebae were always reduced when plants were also infected with mycorrhiza. Microbial biomass increased (¡Á1.4) and microbial growth was less limited by phosphorus in presence of mycorrhiza. However, plant phosphorus uptake did not increase, rather, plant concentrations of carbon and nutrients decreased in presence of mycorrhiza, suggesting a sequestration of resources during the establishment of a mycorrhizal network. Amoebae strongly interacted with, and partly compensated for, the effects of mycorrhiza, demonstrating that interactions between AM fungi and the microbial food web in the rhizosphere significantly feed back on early plant performance.

Keywords: Arbuscular mycorrhiza; Soil protozoa; Root architecture; Plant growth; Microbial interactions

Article Outline
1. Introduction
2. Materials and methods
2.1. Soil and microorganisms
2.2. Plants
2.3. Experimental set-up and analyses
2.4. Statistical analyses
3. Results
3.1. Plants
3.2. Microorganisms
4. Discussion
Acknowledgements
References





Fig. 1. Effects of mycorrhiza and protozoa on root architecture of rice plants: (a) root diameter (mm, +1 S.D.), (b) root volume (cm3, +1 S.D.) and (c) specific root length (SRL; cm g−1, +1 S.D.). See legend of Fig. 3 for treatments and statistics.


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Interactions between arbuscular mycorrhizal fungi (Glomus intraradices, Glomeromycota) and amoebae (Acanthamoeba castellanii, Protozoa) in the rhizosphere of rice (Oryza sativa)
Soil Biology and Biochemistry, Volume 40, Issue 3, March 2008, Pages 660-668
S. Herdler, K. Kreuzer, S. Scheu, M. Bonkowski

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