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分类	共搜索到 149 个相关话题(最多显示前5000个)	作者	最后发表

文献求助	From random to targeted transgene NikolasZeh1,MoritzSchmidt1,PatrickSchulz1,SimonFischer2ThenewfrontierinCHOcelllinedevelopment:FromrandomtotargetedtransgeneintegrationtechnologiesBiotechnolAdv.2024Oct:75:...	xianzhongli	2026-01-19 12:38
文献求助	Programmable promoter editing for ...transgene expression SnehaR.Kabaria,YunbeenBae,MaryE.Ehmann,BrittanyA.Lende-Dorn,AdamM.Beitz,EmmaL.Peterman,KaseyS.Love,DeonS.Ploessl&KateE....	KW	2025-10-15 07:35
文献求助	求助文献一篇 Ji-HuiQiaoetal.Transgene-andtissueculture-freeheritablegenomeeditingusingRNAvirus-baseddeliveryinwheatnatureplants2025Onlineaheadofprinthttps:/doi.org/10.1038/s41477-025-02023-...	st0ne513	2025-06-25 10:05
文献求助	From random to targeted transgene NikolasZeh1,MoritzSchmidt1,PatrickSchulz1,SimonFischer2ThenewfrontierinCHOcelllinedevelopment:FromrandomtotargetedtransgeneintegrationtechnologiesBiotechnolAdv.2024Oct:75:...	知音	2024-09-25 03:48
文献求助	A highly efficient transgene knock-in technology in ... AlexabderG.AllenAhighlyefficienttransgeneknock-intechnologyinclinicallyrelevantcelltypesNaturebiotechnology2023458–469https://www.nature.com/articles/s41587-023-01779-8	zouzongsheng	2024-08-07 03:27
文献求助	switchable transgene system xuewangSpatiotemporalcontrolofgeneexpressionbyalight-switchabletransgenesystemnaturemethods2012266-269https://www.nature.com/articles/nmeth.1892	zouzongsheng	2024-04-28 03:46
文献求助	A highly efficient transgene knock-in technology in ... FengzhangAhighlyefficienttransgeneknock-intechnologyinclinicallyrelevantcelltypesNatBiotechnol2023inpress10.1038/s41587-023-01779-8...	ken19860823	2023-05-04 12:25
文献求助	Targeting the AAVS1 Site by CRISPR/Cas...Transgene Cassette for BrettCromerTargetingtheAAVS1SitebyCRISPR/Cas9withanInducibleTransgeneCassettefortheNeuronalDifferentiationofHumanPluripotentStemCellsMethodsinMolecularBiology2022...	ken19860823	2023-03-21 03:09
文献求助完结子版	In vivo magnetic resonance imaging of transgene expression Weissleder,R.InvivomagneticresonanceimagingoftransgeneexpressionNatMed(2000)6,351-5	slm25404	2022-12-07 08:12
文献求助	p40 bioengineering platform for variant ...transgene express Mr.AdrianWesthaus,Dr.MartiCabanesCreus,Mr.TimoJonker,Mr.ErwanSallard,MissReninaGaleNavarro,Mrs.ErhuaZhu,Mr.GroberBaltazar,Mr.ScottLee,Mr.PatrickWilmott,Dr.AnaiGonzalez-Cordero,...	tshine	2022-03-30 12:01
分子生物	pEASY-Blunt平末端连接，板子上比我脸还干净我使用的是TransGene的pEASY-Blunt载体，感受态用的是生工的目的基因PCR产物为20ul，我取了5ul电泳检测，条带正确然后取PCR产物3ul和vector1ul混合（我怀疑就是目标片段浓度太大，导致我的...	525610427	2021-04-27 02:37
文献求助	Preventing spontaneous genetic ...transgene cassettes of aden MatthewG.CottinghamFionnadhCarrollSusanJ.MorrisAlisonV.TurnerAislingM.VaughanMelissaC....	arielyi	2020-05-27 04:45
文献求助	Carbon nanotube–mediated DNA delivery without transgene ... GozdeS.Demirer,HuanZhang,NatalieS.Goh,EduardoGonzález-Grandío&MarkitaP.LandryCarbonnanotube–mediatedDNAdeliverywithouttransgeneintegrationinintactplantsNatureProtocols201914...	残笙	2019-09-25 08:26
文献求助	求助文献Carbon nanotube–mediated ...transgene integration in GozdeS.Demirer,HuanZhang,NatalieS.Goh,EduardoGonzález-GrandíoCarbonnanotube–mediatedDNAdeliverywithouttransgeneintegrationinintactplantsnatureprotocols20191-45doi.org/10....	jackiey2	2019-09-22 02:36
文献求助	High Efficiency Transgene Segregation in Co-Transformed ... MichaelMiller,LauraTaglianiNingWangBenjaminBerkaDennisBidneyZuo-YuZhaoHighEfficiencyTransgeneSegregationinCo-TransformedMaizePlantsusinganAgrobacteriumTumefaciens2T-...	billxj20	2019-02-07 03:46
文献求助	A murine transmembrane tumor necrosis factor(TNF)transgene ... AlexopoulouL1,PasparakisM,KolliasG.Amurinetransmembranetumornecrosisfactor(TNF)transgeneinducesarthritisbycooperativep55/p75TNFreceptorsignalingEurJImmunol.;...	wayky	2018-12-03 08:08
文献求助	Efficient generation of transgene-and feeder-free induced ... XiaobingTanabQingliDaicTaoGuodJingshuXuabQingyuanDaidEfficientgenerationoftransgene-andfeeder-...	chenqiberg	2018-11-15 02:00
文献求助	Revealing key determinants of clonal variation in transgene ... JaeSeongLee,JinHyoungPark,TaeKwangHa,MojtabaSamoudi,NathanLewis,Bernhard?.Palsson,HeleneFaustrupKildegaard,...	痞子无敌	2018-11-06 03:29
文献求助	求助 Nat Plants一篇Tough GC beats transgene silencing. ZhangXToughGCbeatstransgenesilencing.NatPlants20173(11):850-851doi:10.1038/s41477-017-0048-y.其它外文期刊	huochenmin	2018-08-30 12:27
硕博家园	克隆载体连接不上，菌检非阳性，崩溃边缘片段大小是1000多bp和2000bp，调整过插入片段比，连接时间是25度15分钟和20分钟，一开始用的是transgene的T载体，后来用了takara的in-fusion克隆，结果挑菌还是没有，真的不知道该怎么办了...	yinzqllll	2018-05-23 12:31
文献求助	文献求助 TransgeneExpressionReducesFratricideandPermits4-1BBCostimulationofCARTCellsDirectedtoT-CellMalignancies.info'.2.'][info3]CancerImmunolRes.info'.3.'][info4]...	yafen0628	2017-11-29 01:03
绿色求助(高悬赏)	like,small RNA-mediated transgene si transposon-like,smallRNA-mediatedtransgenesilencinginfo'.2.'][info3]NaturePlantsinfo'.3.'][info4]2017info'.4.'][info5]NaturePlants3,875–884(2017)info'.5.'][info...	54808455	2017-11-13 08:24
文献求助完结子版	文献一片 Leideninfo'.1.'][info2]Immuneresponsestotransgene-encodedproteinslimitthestabilityofgeneexpressionafterinjectionofreplication-defectiveadenovirusvectorsinfo...	youcai147	2017-11-10 07:26
文献求助	文献求助 H.info'.1.'][info2]Aconditionaltissue-specifictransgeneexpressionsystemusinginducibleGAL4.info'.2.'][info3]Proc.Natl.Acad.Sci.USAinfo'.3.'][info4](2001).info'....	乖乖志	2017-11-07 02:32
农林	[优秀文章推荐]转基因植物中外源基因沉默的机制与防止措施转基因沉默(Transgenesilencing)是指导入并整合进受体基因组中的外源基因在转化体的当代或其后代中表达受到抑制的现象，然而在植物中造成转基因沉默现象的机理尚未明确，该研究团队通过...	晋鹏	2017-11-01 06:26