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RNase is not the problem!
The resulting DNA is highly contaminated with high level of secondary materials, such as high levels of polysaccharides, polyphenols and other unknown sticky compounds that bind or co-precipitate with DNA and form a viscous complex. It is not possible to load such DNA samples into the electrophoresis well.
You could try this protocol, which works well on plant total DNA extraction.
3% CTAB DNA extracion protocol
(modified from Zeng et al. 2002, ACta Botanica Sinica 44; 694-697)
1. washing out polysaccharides with CTAB-free buffer from homogenate.
2. increasing the percentage of CTAB in extraction medium.
3. using high concentration salts pior to DNA precipitation with isopanol.
![RNase RNA酶似乎不管用啊，求大神帮忙分析]() |
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