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shellyxss

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[求助] 提交全基因组序列已有1人参与

各位大神我在提交了全基因组序列以后 NCBI给我回复了这样一个文件这是什么意思啊应该怎么办呢

[] We ran your sequences through our Contamination Screen. The screen found
contigs that need to be trimmed and/or excluded. Please adjust the
sequences appropriately and then resubmit your sequences. After you remove the
contamination, trim any Ns at the ends of the sequence and remove any sequences
that are shorter than 200 nt and not part of a multi-component scaffold.

Note that hits in eukaryotic genomes to mitochondrial sequences can be ignored
when specific criteria are met. Those criteria are explained below.

Note that mismatches between the name of the adaptor/primer identified in the screen
and the sequencing technology used to generate the sequencing data should not be used
to discount the validity of the screen results as the adaptors/primers of many
different sequencing platforms share sequence similarity.

[] Some of the sequences hit primers or adaptors used in Illumina or
454 or other sequencing strategies or platforms. Primers at the
end of a sequence should be removed. However, if primers are
present within sequences then you should strongly consider
splitting the sequences at the primers because the primer sequence
could have been the region of overlap, causing a misassembly.

Skipped 1 same as before; no new sequences to screen.
1 sequence with locations to mask/trim

Trim:
Sequence name, length, span(s), apparent source
Scaffold1_1 3953201 1405766..1405795 adaptor:NGB00733.1-not_cleaned

73D1A844-1EF1-468D-8FEE-E143F2F5F31F.png

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Always be curious about nature

1楼 2015-12-19 22:28:04

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

shellyxss

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After you remove the
contamination, trim any Ns at the ends of the sequence and remove any sequences
that are shorter than 200 nt and not part of a multi-component scaffold.

这一段话我不太明白是什么意思