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xujian568

至尊木虫 (著名写手)

Count XU

【答案】应助回帖

详细解释见:
Ballesteros JA, Weinstein, H (1995) Integrated methods for the construction of three dimensional models and computational probing of structure-function relations in G- protein coupled receptors. Methods in Neurosciences 25:366-428
活着就要让人感受到你的存在。
11楼2015-10-27 14:52:34
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天才化学家

新虫 (初入文坛)

这位大神全英文谁看的懂,这个网站全都是英文的吗

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12楼2015-10-27 14:58:50
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天才化学家

新虫 (初入文坛)

求视频教学,还有文献地址

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13楼2015-10-27 14:59:36
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djbitter

新虫 (小有名气)

原文中有说明:
Site-directed mutagenesis. pcDNA3.1+ plasmids containing the human CCR2 mutants I208A5.45 and E291A7.39 (superscript indicates the Ballesteros Weinstein numbering system (Ballesteros, 1995), in which transmembrane residues are assigned two numbers that belong to the helix number and the residue number relative to the most conserved residue in this helix, which is assigned 50) with a 3x hemagglutinin (HA) epitope tag at the N-terminus were kindly provided by James Pease (Hall et al., 2009).
14楼2015-10-27 17:08:09
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djbitter

新虫 (小有名气)

引用回帖:
5楼: Originally posted by 清泉765 at 2015-10-27 11:56:16
比如这张图片里面表示的,前面的数字才是表示氨基酸位置的,我问的是那个上标表示的意思?

图片3.png
...

原文中有说明:Site-directed mutagenesis. pcDNA3.1+ plasmids containing the human CCR2 mutants I208A5.45 and E291A7.39 (superscript indicates the Ballesteros Weinstein numbering system (Ballesteros, 1995), in which transmembrane residues are assigned two numbers that belong to the helix number and the residue number relative to the most conserved residue in this helix, which is assigned 50) with a 3x hemagglutinin (HA) epitope tag at the N-terminus were kindly provided by James Pease (Hall et al., 2009).
15楼2015-10-27 17:09:39
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迷情八月

金虫 (正式写手)

这个应该是距离某中心位点的距离,单位是埃米,Å
16楼2015-10-29 10:48:36
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