±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 3939  |  »Ø¸´: 15

xujian568

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

Count XU

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

Ïêϸ½âÊͼû£º
Ballesteros JA, Weinstein, H (1995) Integrated methods for the construction of three dimensional models and computational probing of structure-function relations in G- protein coupled receptors. Methods in Neurosciences 25:366-428
»îמÍÒªÈÃÈ˸ÐÊܵ½ÄãµÄ´æÔÚ¡£
11Â¥2015-10-27 14:52:34
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

Ìì²Å»¯Ñ§¼Ò

гæ (³õÈëÎÄ̳)

Õâλ´óÉñȫӢÎÄË­¿´µÄ¶®£¬Õâ¸öÍøÕ¾È«¶¼ÊÇÓ¢ÎĵÄÂð

·¢×ÔСľ³æIOS¿Í»§¶Ë
12Â¥2015-10-27 14:58:50
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

Ìì²Å»¯Ñ§¼Ò

гæ (³õÈëÎÄ̳)

ÇóÊÓÆµ½Ìѧ£¬»¹ÓÐÎÄÏ×µØÖ·

·¢×ÔСľ³æIOS¿Í»§¶Ë
13Â¥2015-10-27 14:59:36
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

djbitter

гæ (СÓÐÃûÆø)

Ô­ÎÄÖÐÓÐ˵Ã÷£º
Site-directed mutagenesis. pcDNA3.1+ plasmids containing the human CCR2 mutants I208A5.45 and E291A7.39 (superscript indicates the Ballesteros Weinstein numbering system (Ballesteros, 1995), in which transmembrane residues are assigned two numbers that belong to the helix number and the residue number relative to the most conserved residue in this helix, which is assigned 50) with a 3x hemagglutinin (HA) epitope tag at the N-terminus were kindly provided by James Pease (Hall et al., 2009).
14Â¥2015-10-27 17:08:09
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

djbitter

гæ (СÓÐÃûÆø)

ÒýÓûØÌû:
5Â¥: Originally posted by ÇåȪ765 at 2015-10-27 11:56:16
±ÈÈçÕâÕÅͼƬÀïÃæ±íʾµÄ£¬Ç°ÃæµÄÊý×Ö²ÅÊDZíʾ°±»ùËáλÖõģ¬ÎÒÎʵÄÊÇÄǸöÉϱê±íʾµÄÒâ˼£¿

ͼƬ3.png
...

Ô­ÎÄÖÐÓÐ˵Ã÷£ºSite-directed mutagenesis. pcDNA3.1+ plasmids containing the human CCR2 mutants I208A5.45 and E291A7.39 (superscript indicates the Ballesteros Weinstein numbering system (Ballesteros, 1995), in which transmembrane residues are assigned two numbers that belong to the helix number and the residue number relative to the most conserved residue in this helix, which is assigned 50) with a 3x hemagglutinin (HA) epitope tag at the N-terminus were kindly provided by James Pease (Hall et al., 2009).
15Â¥2015-10-27 17:09:39
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÃÔÇé°ËÔÂ

½ð³æ (ÕýʽдÊÖ)

Õâ¸öÓ¦¸ÃÊǾàÀëijÖÐÐÄλµãµÄ¾àÀ룬µ¥Î»Êǰ£Ã×£¬Å
16Â¥2015-10-29 10:48:36
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ ÇåȪ765 µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 289Çóµ÷¼Á +8 ÐÂʱ´ú²ÄÁÏ 2026-03-27 8/400 2026-03-28 04:09 by fmesaito
[¿¼ÑÐ] 0703»¯Ñ§ +6 ÄÝÄÝninicgb 2026-03-27 6/300 2026-03-27 17:18 by shangxh
[¿¼ÑÐ] 292Çóµ÷¼Á +13 ¶ì¶ì¶ì¶î¶î¶î¶î¶ 2026-03-25 14/700 2026-03-27 15:40 by caszguilin
[¿¼ÑÐ] 305Çóµ÷¼Á +5 ÍÛ¬¿¨¿â 2026-03-26 5/250 2026-03-27 14:01 by laoshidan
[¿¼ÑÐ] 312Çóµ÷¼Á +9 Éϰ¶°ÉZJY 2026-03-22 13/650 2026-03-27 11:24 by sanrepian
[¿¼ÑÐ] 315µ÷¼Á +4 0860Çóµ÷¼Á 2026-03-26 5/250 2026-03-27 11:23 by wangjy2002
[¿¼ÑÐ] ²ÄÁÏÇóµ÷¼Á +5 .m.. 2026-03-25 5/250 2026-03-27 11:08 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] Ò»Ö¾Ô¸¼ª´ó071010£¬316·ÖÇóµ÷¼Á +3 xgbiknn 2026-03-27 3/150 2026-03-27 10:36 by guoweigw
[¿¼ÑÐ] Çóµ÷¼Á +6 ÁÖ֮Ϧ 2026-03-24 6/300 2026-03-27 08:38 by hypershenger
[¿¼ÑÐ] ²ÄÁÏѧ˶£¬Çóµ÷¼Á 6+5 ÌǺù«888ll 2026-03-22 10/500 2026-03-27 08:18 by hypershenger
[¿¼ÑÐ] Ò»Ö¾Ô¸Ö£ÖÝ´óѧ£¬080500ѧ˶£¬×Ü·Ö317·ÖÇóµ÷¼Á +4 ¾Ù¸öÀõ×Óoi 2026-03-24 5/250 2026-03-26 23:15 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] 085602»¯Ñ§¹¤³ÌÇóµ÷¼Á¡£ +4 ƽÀÖÀÖÀÖ 2026-03-26 4/200 2026-03-26 17:57 by fmesaito
[¿¼ÑÐ] ×Ü·Ö293Çóµ÷¼Á +6 ¼ÓÒ»Ò»¾Å 2026-03-25 8/400 2026-03-26 13:30 by yujianx
[¿¼ÑÐ] ÉúÎïѧѧ˶Çóµ÷¼Á +7 СÑò˯×ÅÁË? 2026-03-23 10/500 2026-03-25 02:24 by Çå·ç·÷Ñï¡£ m
[¿¼ÑÐ] ʳƷר˶ һ־Ը˫һÁ÷ 328 +3 xiaom99 2026-03-21 4/200 2026-03-24 21:20 by lailaisimei
[¿¼ÑÐ] 080500Çóµ÷¼Á +3 zzzzfan 2026-03-24 3/150 2026-03-24 16:38 by barlinike
[¿¼ÑÐ] 344Çóµ÷¼Á +3 desto 2026-03-24 3/150 2026-03-24 10:09 by ²«»÷518
[¿¼ÑÐ] 336»¯¹¤µ÷¼Á +4 Íõ´ó̹1 2026-03-23 5/250 2026-03-23 18:32 by allen-yin
[¿¼ÑÐ] Çóµ÷¼ÁԺУÐÅÏ¢ +6 CX 330 2026-03-21 6/300 2026-03-22 15:25 by ÎÞи¿É»÷111
[¿¼ÑÐ] ²ÄÁÏѧ˶301·ÖÇóµ÷¼Á +7 Liyouyumairs 2026-03-21 7/350 2026-03-21 22:31 by peike
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û