| 查看: 244 | 回复: 2 | |||
| 当前主题已经存档。 | |||
| 【有奖交流】积极回复本帖子，参与交流，就有机会分得作者 daijianfeng 的 7 个金币 | |||
daijianfeng银虫 (小有名气)
|
[交流]
求助:5氟尿嘧啶的液相条件
|
||
|
我现在用HPLC测5-氟尿嘧啶,试了很多条件,效果都不好,出峰时间太早.文献的方法都不可靠。 请问哪位知道5-FU的色谱条件？最好是自己做过的。 还有哪位知道5-FU是否有旋光性？是否有荧光？ 谢谢！ |
回复此楼» 猜你喜欢
招博士
已经有4人回复
-
救命帖
已经有7人回复
-
限项规定
已经有6人回复
-
西南交通大学国家级人才团队2026年博士研究生招生（考核制）—机械、材料、力学方向
已经有3人回复
-
英文综述是否需要润色及查重
已经有5人回复
-
为什么nbs上溴 没有产物点出现呢
已经有9人回复
-
最失望的一年
已经有18人回复
2楼2008-07-17 11:42:51
脂质体
至尊木虫 (知名作家)
- 应助: 205 (大学生)
- 金币: 37294.8
- 红花: 48
- 帖子: 5965
- 在线: 892.3小时
- 虫号: 102799
- 注册: 2005-11-14
- 专业: 药剂学
|
Assay— Mobile phase— Use degassed and filtered water. Standard preparation— Dissolve an accurately weighed quantity of USP Fluorouracil RS in water, and dilute quantitatively, and stepwise if necessary, with water to obtain a solution having a known concentration of about 10 µg per mL. Assay preparation— Transfer an accurately weighed quantity of about 20 mg of Fluorouracil to a 200-mL volumetric flask, dissolve in and dilute with water to volume, and mix. Quantitatively dilute a known volume of this solution with water to obtain a solution having a concentration of about 10 µg per mL. Chromatographic system (see Chromatography 621)—The liquid chromatograph is equipped with a 254-nm detector and a 4-mm × 30-cm column containing packing L1. The flow rate is about 1 mL per minute. Chromatograph the Standard preparation, and record the peak responses as directed for Procedure: the column efficiency is not less than 2500 theoretical plates, and the relative standard deviation for replicate injections is not more than 2.0%. Procedure— Separately inject equal volumes (about 10 µL) of the Standard preparation and the Assay preparation into the chromatograph, record the chromatograms, and measure the responses for the major peaks. Calculate the quantity, in mg, of C4H3FN2O2 in the portion of Fluorouracil taken by the formula: 2C(rU / rS) in which C is the concentration, in µg per mL, of USP Fluorouracil RS in the Standard preparation; and rU and rS are the fluorouracil peak responses obtained from the Assay preparation and the Standard preparation, respectively. |
3楼2008-07-17 14:03:01