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为综合利用紫苏资源，延长紫苏加工的产业链，本研究以紫苏叶为原料，以黄酮含量为考察依据，通过单因素实验和响应面实验确定超声波提取紫苏黄酮的最佳工艺条件。结果表明超声辅助提取紫苏黄酮的最佳工艺条件为：超声温度为70℃、乙醇浓度50%、超声时间30min，在最佳提取条件下黄酮含量为52.1mg/g。其次以大孔树脂的吸附率和解析率为参考，对最佳工艺条件提取的紫苏黄酮进行初步纯化。实验表明D-101大孔树脂对紫苏黄酮具有良好的吸附和解吸性能，经D-101树脂吸附富集后，样品总黄酮含量可达110.235mg/g 。最后以过氧化值（POV)的大小为参照指标，采用碘量法评价初步纯化后的紫苏黄酮提取液对对猪油的抗氧化效果。结果表明紫苏黄酮在一定浓度范围内能有效抑制猪油的氧化。 【关键词】紫苏 黄酮 超声波辅助提取法 大孔树脂 抗氧化 |
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灿灿妹
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爱与雨下: 金币+1 2015-05-08 20:14:34
110147加油(RXMCDM代发): 金币+25, 多谢应助！ 2015-10-04 20:05:31
RXMCDM: 翻译EPI+1 2015-10-04 20:05:35
爱与雨下: 金币+1 2015-05-08 20:14:34
110147加油(RXMCDM代发): 金币+25, 多谢应助！ 2015-10-04 20:05:31
RXMCDM: 翻译EPI+1 2015-10-04 20:05:35
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For comprehensive utilization of perilla resource and lengthening the production chain of perilla process, the optimal technological conditions of perilla flavone extraction using ultrasonic wave were determined through the single factor experiment and response surface experiment based on the flavonoid content. The results showed that the optimal technological conditions were determined as following: ultrasound temperature 70℃, ethanol concentration 50% and ultrasound time 30min, and under which the flavonoid content was 52.1mg/g. Then, the perilla flavone that was extracted at the optimal technological conditions was initial purified by using the adsorption rate and desorption rate of macroporous resin as the reference. Experiment showed the D-101 macroporous resin has a good absorptive property and desorption performance to perilla flavone, after the absorptive enrichment of which, the total flavonoid content of the samples could reach 110.235mg/g. Finally,the antioxidant effect of extracting solution of the initial purified perilla flavone to lard was evaluated based on iodometry. The results showed that perilla flavone could effectively inhibit the oxidation of lard in certain range of concentrations. |
2楼2015-05-08 09:16:40
3楼2015-05-08 13:01:43