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[求助]
Hela 细胞中mRNA的序列已有2人参与
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| 最近在做mRNA的检测，但是找不到Hela细胞的序列，有做过高人知道的么？ |
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4楼2015-01-20 16:34:47
781055707
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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★
感谢参与，应助指数 +1
chinahcp2014: 金币+8, ★★★★★最佳答案 2015-01-21 09:18:10
感谢参与，应助指数 +1
chinahcp2014: 金币+8, ★★★★★最佳答案 2015-01-21 09:18:10
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Dictyostelium fasciculatum putative RNA helicase (helA) mRNA, complete cds NCBI Reference Sequence: XM_004350757.1 FASTA Graphics LOCUS XM_004350757 3231 bp mRNA linear INV 26-MAR-2013 DEFINITION Dictyostelium fasciculatum putative RNA helicase (helA) mRNA, complete cds. ACCESSION XM_004350757 VERSION XM_004350757.1 GI:470237701 KEYWORDS RefSeq. SOURCE Dictyostelium fasciculatum ORGANISM Dictyostelium fasciculatum Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium. REFERENCE 1 (bases 1 to 3231) AUTHORS Gloeckner,G., Schaap,P., Noegel,A.A., Felder,M., Eichinger,L., Heidel,A.J. and Platzer,M. TITLE Living fossils from the dawn of multicellularity JOURNAL Unpublished REFERENCE 2 (bases 1 to 3231) AUTHORS Gloeckner,G., Schaap,P., Noegel,A.A., Felder,M. and Platzer,M. TITLE Direct Submission JOURNAL Submitted (29-JAN-2010) Genome Analysis, Fritz Lipmann Institute, Beutenbergstr. 11, Jena 07745, Germany COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_004457624). COMPLETENESS: incomplete on both ends. FEATURES Location/Qualifiers source 1..3231 /organism="Dictyostelium fasciculatum" /mol_type="mRNA" /strain="SH3" /db_xref="taxon:261658" /chromosome="Unknown" gene <1..>3231 /gene="helA" /locus_tag="DFA_11864" /db_xref="GeneID:14865676" CDS 1..3231 /gene="helA" /locus_tag="DFA_11864" /codon_start=1 /product="putative RNA helicase" /protein_id="XP_004350808.1" /db_xref="GI:470237702" /db_xref="GeneID:14865676" /translation="MQMAGGKPKPTTSSGSNANVKIKNKENQGKNDKQNKYLNQKKKE SKTGNLKYLPNKKDDKKDKPVAPGSTLLQGTQDLKAPEKGWSVHSSFFNQDFLKGKAA DKHSQFESSANKFNFNIDDHSDGDDNQELDDNYDTNLFADVGGFNLQDDEGMLDDDDF DLLDYDDEALEDHEGEAREAKAIAEEGGVFFEDQYSLSVEEKMQERKKKIDQSFKTKD DDLLKSFPSIGREDEDGKTVSMDNDDEMDEDAKKVARLTDIQDERKKKMGGFQGMDLH KFLFKAVMKKGFKQPTPIQRLTIPLILEGQDVVGMARTGSGKTAAFVIPMIQKLAQHS HKVGARAIILSPTRELALQTYRVVKELSSGSDLRSCVIVGGDNMADQFTELARNPDII IATPGRLVHHLTEVNMGLHTVQYIVFDEADRLFEMGFADQLQEIITKLSPSRQTLLFS ATLPSMLAEFVRAGLSNPKLVRLNTDTKISDQLSLSFFTCRHDEKFAALLYLLKDIIK DDEPTIVFTATKFHVEYLHILLDQARIANTFIHGQMDPVARKINLAKFRSKVVNVMVV TDLAARGIDIPMLDNVINFDFAPKEKIFIHRVGRVARAGRKGTAYSLVSPDEIPYMLD LHLYIAKKLTNKLLPGQTVEDLEYSFYGNIPQQIIDRENEFVAIKAKECVELLSLKRT IFNAHGKYVRTRPGASHESNKRAKGLDKSQYHPLMYNFINSNEAVRNDFVQSLKAFRP NQTVFEMTGKKSSAADIMQTKREFHSTVIGQQHRRTQAEIEEYKKQDQLNRNSEDGEE AASTKKNSKRKRDKNEDDEGFLQELEQNTKQAAEEENGKKKQKGHIIDENEQEEGDDQ VKYKITIDDSESKAAELKNSKKKTRSSTKDEAFFMSSTPSNYYQERALAITDKFTRDD EVNMGTDDGQNKTPGGSSGKKWDRKKKKFVSVQADADREKERKKWVRNEAGVLVDTSK NKKNLYEEWKKKTNSRIQRTGEEENARFMPAKPTYVPVKWRGNKQQAAEGEDGKKKNI RTGIKDIDDVRKERDRRDHHSKIKHTSATKNKFNKGKGGKGGKKRK" ORIGIN 1 atgcaaatgg caggaggtaa accaaaacct actactagta gtggtagtaa tgccaatgta 61 aagataaaga ataaagagaa tcaaggaaag aatgataaac aaaacaaata cttgaatcaa 121 aagaaaaagg aatcaaagac tggcaacctc aagtacttgc ccaacaaaaa ggacgataaa 181 aaggacaagc ctgtagcacc gggcagcaca ttgttgcaag gtacacaaga cctcaaggca 241 cccgagaagg gatggagtgt gcacagctca ttctttaacc aagatttcct caagggcaag 301 gctgccgaca aacacagtca attcgaatcg agtgccaaca agtttaactt taacattgac 361 gatcatagcg acggcgacga caaccaagag ttggacgaca actatgacac taatctcttt 421 gccgacgtcg gcgggttcaa cctgcaagac gacgagggta tgttggacga cgacgacttt 481 gatttgttgg actatgacga cgaagcattg gaggatcacg aaggtgaggc tcgtgaagcc 541 aaggctatcg ccgaagaggg tggtgtgttc tttgaagacc agtactcctt gtcggtggag 601 gaaaagatgc aagagcgtaa gaaaaagatc gaccagtcgt tcaagacaaa ggacgacgac 661 ttgctcaaga gtttcccatc aattggcagg gaagatgagg acggcaagac cgtgtcgatg 721 gacaacgacg acgagatgga cgaggacgcc aaaaaggttg cgcgtctcac cgatattcaa 781 gacgagcgca agaagaagat gggtgggttc cagggtatgg acctgcacaa gttcctcttc 841 aaggccgtca tgaaaaaggg gttcaagcaa cccaccccta ttcaaagact caccatccca 901 ctcatcctcg aaggtcaaga tgtcgttggt atggcacgta ccggttccgg taagactgcc 961 gctttcgtca ttccaatgat tcaaaagctc gcacaacatt ctcacaaggt tggtgcacgt 1021 gccatcatcc tctcgcccac tcgtgaactt gctctccaga cataccgtgt cgtcaaggaa 1081 ttgtcgagtg gatctgatct cagatcgtgc gtcattgtcg gtggtgacaa tatggccgac 1141 cagtttacag agctcgctag aaaccccgat atcatcattg ccactccagg tcgtcttgtc 1201 catcatctca ccgaagtcaa catgggtctc cacactgtcc aatacattgt attcgatgaa 1261 gctgatcgtc tctttgaaat gggttttgcc gatcaattgc aagaaatcat taccaaactt 1321 tccccatccc gtcaaaccct tctcttttct gctacccttc catccatgct cgctgaattt 1381 gttcgtgcag gtttatctaa tcctaaatta gttagattaa atactgatac caaaatttct 1441 gatcaattat ctttatcatt ctttacatgt agacatgatg aaaaatttgc agcattactt 1501 tatttattaa aagatattat taaagatgat gaaccaacga ttgtatttac agccacaaaa 1561 ttccatgtcg aatatttaca tatacttttg gatcaagctc gtattgccaa tacgtttatt 1621 cacggtcaga tggatccagt tgctagaaag atcaatcttg ccaagttccg ttccaaggtg 1681 gtcaatgtca tggtggtaac cgatcttgcc gctcgtggta tcgatatccc catgttggac 1741 aatgtcatta atttcgactt tgcacccaag gaaaagatct ttattcacag agttggtcgt 1801 gttgctagag ctggtcgtaa gggtactgcc tactcactcg tctctccaga cgaaatacca 1861 tacatgttgg atctccatct ctacattgcc aagaaactca caaacaaatt gttgccaggt 1921 caaactgtcg aagatttgga atactctttc tatggaaata tccctcaaca aattattgat 1981 cgtgaaaatg aatttgttgc tattaaagct aaagaatgtg ttgaattgtt atcattaaaa 2041 agaactatat ttaatgcaca tggaaaatat gttagaacta gaccaggagc atcacatgaa 2101 tcaaataagc gtgccaaggg attggacaag tcacagtacc atccattgat gtacaatttt 2161 atcaattcca acgaagctgt ccgtaacgac tttgtacagt cactcaaagc attccgtcca 2221 aaccaaaccg tctttgaaat gaccggtaaa aagagctctg ccgctgatat catgcagaca 2281 aagagagagt tccactcgac ggtcattggc caacaacacc gtcgtacaca ggctgaaatc 2341 gaagagtaca agaaacaaga tcaattaaat agaaacagcg aagatggtga agaagctgct 2401 agtaccaaaa agaacagcaa gagaaagaga gacaaaaacg aagacgatga aggattttta 2461 caagagttgg aacaaaacac taaacaagcg gccgaagagg agaatggtaa aaagaaacaa 2521 aagggtcata ttatcgacga aaacgaacaa gaggaaggtg acgaccaagt taaatacaag 2581 attacaattg atgattcaga gtcaaaggct gcagaattga aaaatagcaa gaaaaagact 2641 agatcatcga ccaaggacga agcattcttt atgtcatcga caccatccaa ctactatcaa 2701 gaacgtgcac ttgccatcac tgacaagttt acacgtgacg atgaagtcaa tatgggtacc 2761 gacgatggac aaaacaaaac acctggaggg tcgtctggca aaaagtggga cagaaagaaa 2821 aagaagtttg tcagtgtaca agccgacgcc gacagagaaa aggaacgcaa gaaatgggtc 2881 agaaacgaag ctggtgtact tgtcgacaca tcgaaaaaca aaaagaactt atacgaggaa 2941 tggaagaaga agaccaactc gagaattcaa cgtaccggag aagaagaaaa cgctagattc 3001 atgcctgcca aaccaaccta tgtccccgtc aaatggagag gtaacaaaca acaagcagcc 3061 gaaggtgaag atggtaaaaa gaaaaatatt cgtactggta tcaaagatat cgatgatgtc 3121 agaaaggaac gtgatagaag agatcatcat agcaagatta aacatacttc tgccactaaa 3181 aacaaattca acaaaggtaa aggtggtaaa ggtggtaaaa agagaaaata a // |
2楼2015-01-20 12:19:23
781055707
木虫 (著名写手)
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3楼2015-01-20 12:21:44
5楼2015-01-20 16:49:40