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chinahcp2014

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[求助] Hela 细胞中mRNA的序列已有2人参与

最近在做mRNA的检测，但是找不到Hela细胞的序列，有做过高人知道的么？

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1楼2015-01-20 10:52:46

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781055707

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感谢参与，应助指数 +1
chinahcp2014: 金币+8, ★★★★★最佳答案 2015-01-21 09:18:10

Dictyostelium fasciculatum putative RNA helicase (helA) mRNA, complete cds
NCBI Reference Sequence: XM_004350757.1
FASTA Graphics
LOCUS    XM_004350757          3231 bp mRNA linear INV 26-MAR-2013
DEFINITION  Dictyostelium fasciculatum putative RNA helicase (helA) mRNA,
         complete cds.
ACCESSION XM_004350757
VERSION    XM_004350757.1  GI:470237701
KEYWORDS RefSeq.
SOURCE    Dictyostelium fasciculatum
  ORGANISM  Dictyostelium fasciculatum
         Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
REFERENCE 1  (bases 1 to 3231)
  AUTHORS Gloeckner,G., Schaap,P., Noegel,A.A., Felder,M., Eichinger,L.,
         Heidel,A.J. and Platzer,M.
  TITLE    Living fossils from the dawn of multicellularity
  JOURNAL Unpublished
REFERENCE 2  (bases 1 to 3231)
  AUTHORS Gloeckner,G., Schaap,P., Noegel,A.A., Felder,M. and Platzer,M.
  TITLE    Direct Submission
  JOURNAL Submitted (29-JAN-2010) Genome Analysis, Fritz Lipmann Institute,
         Beutenbergstr. 11, Jena 07745, Germany
COMMENT    PROVISIONAL REFSEQ: This record has not yet been subject to final
         NCBI review. This record is derived from an annotated genomic
         sequence (NW_004457624).
         COMPLETENESS: incomplete on both ends.
FEATURES          Location/Qualifiers
   source       1..3231
                  /organism="Dictyostelium fasciculatum"
                  /mol_type="mRNA"
                  /strain="SH3"
                  /db_xref="taxon:261658"
                  /chromosome="Unknown"
   gene          <1..>3231
                  /gene="helA"
                  /locus_tag="DFA_11864"
                  /db_xref="GeneID:14865676"
   CDS          1..3231
                  /gene="helA"
                  /locus_tag="DFA_11864"
                  /codon_start=1
                  /product="putative RNA helicase"
                  /protein_id="XP_004350808.1"
                  /db_xref="GI:470237702"
                  /db_xref="GeneID:14865676"
                  /translation="MQMAGGKPKPTTSSGSNANVKIKNKENQGKNDKQNKYLNQKKKE
                  SKTGNLKYLPNKKDDKKDKPVAPGSTLLQGTQDLKAPEKGWSVHSSFFNQDFLKGKAA
                  DKHSQFESSANKFNFNIDDHSDGDDNQELDDNYDTNLFADVGGFNLQDDEGMLDDDDF
                  DLLDYDDEALEDHEGEAREAKAIAEEGGVFFEDQYSLSVEEKMQERKKKIDQSFKTKD
                  DDLLKSFPSIGREDEDGKTVSMDNDDEMDEDAKKVARLTDIQDERKKKMGGFQGMDLH
                  KFLFKAVMKKGFKQPTPIQRLTIPLILEGQDVVGMARTGSGKTAAFVIPMIQKLAQHS
                  HKVGARAIILSPTRELALQTYRVVKELSSGSDLRSCVIVGGDNMADQFTELARNPDII
                  IATPGRLVHHLTEVNMGLHTVQYIVFDEADRLFEMGFADQLQEIITKLSPSRQTLLFS
                  ATLPSMLAEFVRAGLSNPKLVRLNTDTKISDQLSLSFFTCRHDEKFAALLYLLKDIIK
                  DDEPTIVFTATKFHVEYLHILLDQARIANTFIHGQMDPVARKINLAKFRSKVVNVMVV
                  TDLAARGIDIPMLDNVINFDFAPKEKIFIHRVGRVARAGRKGTAYSLVSPDEIPYMLD
                  LHLYIAKKLTNKLLPGQTVEDLEYSFYGNIPQQIIDRENEFVAIKAKECVELLSLKRT
                  IFNAHGKYVRTRPGASHESNKRAKGLDKSQYHPLMYNFINSNEAVRNDFVQSLKAFRP
                  NQTVFEMTGKKSSAADIMQTKREFHSTVIGQQHRRTQAEIEEYKKQDQLNRNSEDGEE
                  AASTKKNSKRKRDKNEDDEGFLQELEQNTKQAAEEENGKKKQKGHIIDENEQEEGDDQ
                  VKYKITIDDSESKAAELKNSKKKTRSSTKDEAFFMSSTPSNYYQERALAITDKFTRDD
                  EVNMGTDDGQNKTPGGSSGKKWDRKKKKFVSVQADADREKERKKWVRNEAGVLVDTSK
                  NKKNLYEEWKKKTNSRIQRTGEEENARFMPAKPTYVPVKWRGNKQQAAEGEDGKKKNI
                  RTGIKDIDDVRKERDRRDHHSKIKHTSATKNKFNKGKGGKGGKKRK"
ORIGIN
      1 atgcaaatgg caggaggtaa accaaaacct actactagta gtggtagtaa tgccaatgta
   61 aagataaaga ataaagagaa tcaaggaaag aatgataaac aaaacaaata cttgaatcaa
   121 aagaaaaagg aatcaaagac tggcaacctc aagtacttgc ccaacaaaaa ggacgataaa
   181 aaggacaagc ctgtagcacc gggcagcaca ttgttgcaag gtacacaaga cctcaaggca
   241 cccgagaagg gatggagtgt gcacagctca ttctttaacc aagatttcct caagggcaag
   301 gctgccgaca aacacagtca attcgaatcg agtgccaaca agtttaactt taacattgac
   361 gatcatagcg acggcgacga caaccaagag ttggacgaca actatgacac taatctcttt
   421 gccgacgtcg gcgggttcaa cctgcaagac gacgagggta tgttggacga cgacgacttt
   481 gatttgttgg actatgacga cgaagcattg gaggatcacg aaggtgaggc tcgtgaagcc
   541 aaggctatcg ccgaagaggg tggtgtgttc tttgaagacc agtactcctt gtcggtggag
   601 gaaaagatgc aagagcgtaa gaaaaagatc gaccagtcgt tcaagacaaa ggacgacgac
   661 ttgctcaaga gtttcccatc aattggcagg gaagatgagg acggcaagac cgtgtcgatg
   721 gacaacgacg acgagatgga cgaggacgcc aaaaaggttg cgcgtctcac cgatattcaa
   781 gacgagcgca agaagaagat gggtgggttc cagggtatgg acctgcacaa gttcctcttc
   841 aaggccgtca tgaaaaaggg gttcaagcaa cccaccccta ttcaaagact caccatccca
   901 ctcatcctcg aaggtcaaga tgtcgttggt atggcacgta ccggttccgg taagactgcc
   961 gctttcgtca ttccaatgat tcaaaagctc gcacaacatt ctcacaaggt tggtgcacgt
   1021 gccatcatcc tctcgcccac tcgtgaactt gctctccaga cataccgtgt cgtcaaggaa
   1081 ttgtcgagtg gatctgatct cagatcgtgc gtcattgtcg gtggtgacaa tatggccgac
   1141 cagtttacag agctcgctag aaaccccgat atcatcattg ccactccagg tcgtcttgtc
   1201 catcatctca ccgaagtcaa catgggtctc cacactgtcc aatacattgt attcgatgaa
   1261 gctgatcgtc tctttgaaat gggttttgcc gatcaattgc aagaaatcat taccaaactt
   1321 tccccatccc gtcaaaccct tctcttttct gctacccttc catccatgct cgctgaattt
   1381 gttcgtgcag gtttatctaa tcctaaatta gttagattaa atactgatac caaaatttct
   1441 gatcaattat ctttatcatt ctttacatgt agacatgatg aaaaatttgc agcattactt
   1501 tatttattaa aagatattat taaagatgat gaaccaacga ttgtatttac agccacaaaa
   1561 ttccatgtcg aatatttaca tatacttttg gatcaagctc gtattgccaa tacgtttatt
   1621 cacggtcaga tggatccagt tgctagaaag atcaatcttg ccaagttccg ttccaaggtg
   1681 gtcaatgtca tggtggtaac cgatcttgcc gctcgtggta tcgatatccc catgttggac
   1741 aatgtcatta atttcgactt tgcacccaag gaaaagatct ttattcacag agttggtcgt
   1801 gttgctagag ctggtcgtaa gggtactgcc tactcactcg tctctccaga cgaaatacca
   1861 tacatgttgg atctccatct ctacattgcc aagaaactca caaacaaatt gttgccaggt
   1921 caaactgtcg aagatttgga atactctttc tatggaaata tccctcaaca aattattgat
   1981 cgtgaaaatg aatttgttgc tattaaagct aaagaatgtg ttgaattgtt atcattaaaa
   2041 agaactatat ttaatgcaca tggaaaatat gttagaacta gaccaggagc atcacatgaa
   2101 tcaaataagc gtgccaaggg attggacaag tcacagtacc atccattgat gtacaatttt
   2161 atcaattcca acgaagctgt ccgtaacgac tttgtacagt cactcaaagc attccgtcca
   2221 aaccaaaccg tctttgaaat gaccggtaaa aagagctctg ccgctgatat catgcagaca
   2281 aagagagagt tccactcgac ggtcattggc caacaacacc gtcgtacaca ggctgaaatc
   2341 gaagagtaca agaaacaaga tcaattaaat agaaacagcg aagatggtga agaagctgct
   2401 agtaccaaaa agaacagcaa gagaaagaga gacaaaaacg aagacgatga aggattttta
   2461 caagagttgg aacaaaacac taaacaagcg gccgaagagg agaatggtaa aaagaaacaa
   2521 aagggtcata ttatcgacga aaacgaacaa gaggaaggtg acgaccaagt taaatacaag
   2581 attacaattg atgattcaga gtcaaaggct gcagaattga aaaatagcaa gaaaaagact
   2641 agatcatcga ccaaggacga agcattcttt atgtcatcga caccatccaa ctactatcaa
   2701 gaacgtgcac ttgccatcac tgacaagttt acacgtgacg atgaagtcaa tatgggtacc
   2761 gacgatggac aaaacaaaac acctggaggg tcgtctggca aaaagtggga cagaaagaaa
   2821 aagaagtttg tcagtgtaca agccgacgcc gacagagaaa aggaacgcaa gaaatgggtc
   2881 agaaacgaag ctggtgtact tgtcgacaca tcgaaaaaca aaaagaactt atacgaggaa
   2941 tggaagaaga agaccaactc gagaattcaa cgtaccggag aagaagaaaa cgctagattc
   3001 atgcctgcca aaccaaccta tgtccccgtc aaatggagag gtaacaaaca acaagcagcc
   3061 gaaggtgaag atggtaaaaa gaaaaatatt cgtactggta tcaaagatat cgatgatgtc
   3121 agaaaggaac gtgatagaag agatcatcat agcaagatta aacatacttc tgccactaaa
   3181 aacaaattca acaaaggtaa aggtggtaaa ggtggtaaaa agagaaaata a
//

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2楼2015-01-20 12:19:23

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

781055707

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专业: 生物大分子结构与功能

这段序列全是MRNA.

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3楼2015-01-20 12:21:44

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chinahcp2014

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引用回帖:

2楼: Originally posted by 781055707 at 2015-01-20 12:19:23
Dictyostelium fasciculatum putative RNA helicase (helA) mRNA, complete cds
NCBI Reference Sequence: XM_004350757.1
FASTA Graphics
LOCUS XM_004350757 3231 bp mRNA linear I ...

你好，谢谢你的回复，我这方面知识欠缺，这是一整条链，还是几段啊？