大家好,我投的文章审稿人意见给出,几个审稿人都给出这样的意见类似
1. only one reference gene was used for qPCR analysis, more than two genes are recommended to normalize qPCR gene expression data.
a) two moreadditional housekeeping genes need to be run to calculate mRNA abundance of gene expression;
那么如何该用多个基因来校正,相对荧光定量的结果呢
或者我应该如何来回答审稿人的意见呢?