24小时热门版块排行榜    

查看: 4432  |  回复: 12
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

BioChen

银虫 (正式写手)

[求助] TopHat初学者 Could not find Bowtie 2 index files (genome.*.bt2) 已有1人参与

tophat -p 4 -G genes.gtf -o C1_R1_thout genome GSM794483_C1_R1_1.fq GSM794483_C1_R1_2.fq

[2014-10-22 13:40:45] Beginning TopHat run (v2.0.13)
-----------------------------------------------
[2014-10-22 13:40:45] Checking for Bowtie
                  Bowtie version:         2.2.3.0
[2014-10-22 13:40:45] Checking for Bowtie index files (genome)..
Error: Could not find Bowtie 2 index files (genome.*.bt2)
回复此楼
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

wawa_eva

新虫 (初入文坛)

bowtie2-build genome.fasta index
3楼2014-10-22 15:33:42
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 13 个回答

BioChen

银虫 (正式写手)

引用回帖:
2楼: Originally posted by wawa_eva at 2014-10-22 15:31:08
先用bowtie2建库

我是下载的bt2文件,还需要建库吗?
网上有人说是路径问题,到底要把这些bt2文件放到哪里呢?
4楼2014-10-22 21:51:01
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

wizardfan

至尊木虫 (著名写手)

优秀版主

【答案】应助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感谢参与,应助指数 +1
BioChen: 金币+50, ★★★★★最佳答案, 谢谢帮助。我了个去,居然最后的原因是文件没有拷贝完全,我把下载的index文件夹里面全部文件拷贝之后,没有问题了。 2014-10-23 14:03:26
你下载了几个bt2文件?名字是什么?
根据manual
The basename of the genome index to be searched. The basename is the name of any of the index files up to but not including the first period. Bowtie first looks in the current directory (第一看当前目录) for the index files, then looks in the indexes subdirectory (第二看bowtie所在目录的indexes子目录) under the directory where the currently-running bowtie executable is located, then looks in the directory specified in the BOWTIE_INDEXES  (or BOWTIE2_INDEXES) environment variable(或者设置BOWTIE2_INDEXES环境变量).
Please note that it is highly recommended that a FASTA file with the sequence(s) the genome being indexed be present in the same directory with the Bowtie index files and having the name <genome_index_base>.fa. If not present, TopHat will automatically rebuild this FASTA file from the Bowtie index files.
5楼2014-10-23 06:58:45
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

七叔118

新虫 (初入文坛)

解决了吗?我也遇到同样问题,bowtie2-build genome.fasta index也跑了,但还是提示找不到,生成的bt2文件需要放到什么位置?
6楼2015-05-12 09:00:23
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[论文投稿] 售SCI一区T0P文章,我:8.O.55.1.O.5.4,科目全,可+急 +3 QTy3jDtz1uLt 2026-08-21 9/450 2026-08-23 03:04 by OEbVnUOu01ol
[硕博家园] 售SCI一区T0P文章,我:8O.55.1.O.54,科目全,可伽急 +4 QTy3jDtz1uLt 2026-08-21 11/550 2026-08-23 02:55 by OEbVnUOu01ol
[考博] 售SCI文章,我:8O.5.5.1O.54,科目全,可十急 +3 DpSrDtM079iu 2026-08-22 3/150 2026-08-23 00:55 by OEbVnUOu01ol
[硕博家园] 售SCI一区文章,我:8O5.5.1.O5.4,科目全,可伽急 +3 2JOx3r2CYEgw 2026-08-22 6/300 2026-08-23 00:40 by OEbVnUOu01ol
[硕博家园] 售SCI一区T0P文章,我:8.O.55.1.O54,科目全,可伽急 +3 2JOx3r2CYEgw 2026-08-22 3/150 2026-08-22 22:03 by OEbVnUOu01ol
[基金申请] 93BebMhtakh前后11位开头都是大写 +7 且听虎啸 2026-08-17 8/400 2026-08-22 21:55 by 医学老男孩
[教师之家] 跳槽后在研项目怎么办? +3 简单化xn 2026-08-22 7/350 2026-08-22 19:46 by 简单化xn
[基金申请] 什么时候开奖? +7 CrisMessi 2026-08-18 8/400 2026-08-22 18:29 by 淀粉搬运工
[基金申请] 只有每年这种时候来逛逛小木虫 +24 yaoyewhu2008 2026-08-20 26/1300 2026-08-22 17:43 by kammury
[基金申请] filecode,4个jtjc了 +13 ziyangfang 2026-08-19 16/800 2026-08-22 17:08 by WH3796
[基金申请] 今天基金会出结果吗?20260819 +16 kkkl_v 2026-08-19 17/850 2026-08-22 16:12 by 阿布Abu
[基金申请] 放榜前的不淡定 40+4 snowwithsea 2026-08-19 14/700 2026-08-21 23:51 by cratir
[基金申请] 建议基金发布提前给出明确的时间点 +10 kulium 2026-08-21 13/650 2026-08-21 21:58 by alongwaytogo
[基金申请] 科研孤儿太难了 +17 我4大白菜 2026-08-20 18/900 2026-08-21 20:57 by zhangev
[基金申请] 看来今天不会放榜了? +8 chengyan1220 2026-08-21 11/550 2026-08-21 17:52 by dcqxinyang
[基金申请] 时间戳又变了 +13 wuchongjun 2026-08-20 19/950 2026-08-21 17:21 by 紫杉醇
[基金申请] 感觉是下周放榜了 +7 angus9576 2026-08-17 12/600 2026-08-21 13:38 by weiyin
[论文投稿] 投稿咨询 +5 wwm09 2026-08-17 7/350 2026-08-21 10:11 by 期刊论文帮手
[基金申请] 明天放榜? +5 Shxjjxjkx 2026-08-18 5/250 2026-08-18 18:14 by -大大大大大-
[基金申请] 今天维护系统维护 祝所有人 高中 +8 gjjjzhong 2026-08-18 9/450 2026-08-18 13:01 by 家与远方
信息提示
请填处理意见