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maoxixi

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[求助] 这两轮PCR用的引物一样吗?已有1人参与

The designer primers, consisting of the
pathway-specific sequences for the targeted classes of natural products
and unbiased AT-rich tags, are summarized in Table 1. The pathwayspecific
PCR primers for type I PKSs,33 type II PKSs,34 NRPSs,33 and
DTSs35 were similarly designed according to literature protocols
(Figure S2A), and an 18 bp AT-rich tag sequence was added at the 5′
end of each pathway-specific primer to form the designer primers for
the first round of PCR. The primers for the second round of PCR
were identical to the 18 bp AT-rich tag sequences.

这两轮PCR用的引物一样吗?求解答。。。
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maoxixi: 金币+5, 有帮助 2014-10-28 20:24:12
没有做过类似的实验,不过按照字面意思是不一样呃,模板的AT含量丰富,所以在一般PCR扩增的条件下增加了一步针对AT的引物扩增
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2楼2014-10-14 20:40:23
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