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Romaner007

金虫 (小有名气)

应助: 4 (幼儿园)
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虫号: 2117057
注册: 2012-11-09
性别: GG
专业: 细胞增殖、生长与分化

[求助] 求助关于shRNA的问题，做基因表达的围观一下吧，给点建议

atggctcgc atcaatcgtc ctgctccagt ggaggtttgttacaagaaca tgcgcttcct gatcacccac aatcccacca atgcaacaat gaataccttc atagaggatc tgaagaaata tggcgccacc acggtggtga gggtgtgcga gattacgtac gacaagacgc ccctagagaa ggacggcatc actgtaatgg actggccgtt cgatgatggg gctccacctc ccaataaaat tgtcgatgat tggttgaatt tactgaagac gaaattctgt gaagatcctg gctgctgcgt agctgttcat tgtgtggccg gtttgggaag ggcgccggtc ctggtcgctc ttgcacttat cgagagtggg atgaaatatg aagatgccat tcagttcatc agacagaagc gccggggggc cattaacagc aagcagctga cgtacctgga gaaataccgg ccgaagcaga ggctacgatt caaggatccg  cacaatcaca  agaacaaatg  ctgcatcatg taa

CDS of gene X002 includes start code ATG and stop code TAA above. The sequence was analised by Block-It online and best target sequences have been suggested as:
GCGAGATTACGTACGACAA
GCTGACGTACCTGGAGAAA
Please take one and design shRNA depending on vector pSilencer 3.1 for knocking-down the gene expression in stable pattern after transfection. The loop sequence between sense and anti-sense is: TTCAAGAGA. The required vector is linearized with sticky ends and is ready for use. If you have any more questions please ask me without hesitation.

BamHI GGATCC,
      CCTAGG

HinDIII AAGCTT
      TTCGAA

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