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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ laitianmao: 金币+11, ★★★★★最佳答案 2014-09-08 14:26:55
Description Back to Top
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). Lambda DNA is 48,502 base pairs in length.
Source
The phage is isolated from the heat-inducible lysogen E. coli l cI857 S7. The DNA is isolated from the purified phage by phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0), 1 mM EDTA.
Properties and Usage Back to Top
Storage Temperature
-20°C
Storage Conditions
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
References Back to Top
Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R. A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
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