| ²é¿´: 4478 | »Ø¸´: 6 | |||
| µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû | |||
061719гæ (СÓÐÃûÆø)
|
[ÇóÖú]
¼×»ù»¯DNAÃâÒß¹²³Áµí-ʵʱ¶¨Á¿PCR·¨(MeDIP-qPCR) ÒÑÓÐ2È˲ÎÓë
|
||
|
ÇëÎʸ÷룬ÓÐÈË×ö¹ýÕâ¸öô£¿·ÑÓÃÊÇÔõÑùµÄ£¿ ÓÃÕâÖÖ·½·¨Ñé֤оƬ£¬ÊÇ·ñ¿ÉÐУ¿ |
» ²ÂÄãϲ»¶
ºþ±±Ê¦·¶´óѧÕе÷¼ÁÀ²
ÒѾÓÐ9È˻ظ´
ÉÛÑôѧԺʳƷÓ뻯ѧ¹¤³ÌѧԺ˶ʿµ÷¼Á
ÒѾÓÐ0È˻ظ´
»¯Ñ§¹¤³Ì¼°¹¤Òµ»¯Ñ§ÂÛÎÄÈóÉ«/·ÒëÔõôÊÕ·Ñ?
ÒѾÓÐ102È˻ظ´
Ìì½òÉÌÒµ´óѧ ÉúÎïÓëÒ½Ò©¿ÎÌâ×éÕÐÉú
ÒѾÓÐ0È˻ظ´
ÉúÎﻯ¹¤Ñ§Ë¶ÕÐÊÕµ÷¼Á
ÒѾÓÐ0È˻ظ´
¹ã¶«Ê¯ÓÍ»¯¹¤Ñ§Ôº2026Äê˶ʿÑо¿ÉúÐèÒª¶àÃûÉúÎï£¬ÖÆÒ©¹¤³Ì£¬Ê³Æ·×¨ÒµµÄµ÷¼ÁÉú
ÒѾÓÐ0È˻ظ´
»¯¹¤É격
ÒѾÓÐ3È˻ظ´
É격×Ô¼ö
ÒѾÓÐ3È˻ظ´
¹ãÎ÷Ãñ×å´óѧ»¯Ñ§»¯¹¤Ñ§Ôº»¯Ñ§¹¤³ÌÓë¼¼Êõѧ˶£¬²ÄÁÏ»¯¹¤×¨Ë¶µ÷¼ÁÃû¶î³ä×㣡
ÒѾÓÐ0È˻ظ´
» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:
ÎÊһϹØÓÚÃâÒß¹²³ÁµíµÄÎÊÌâ
ÒѾÓÐ5È˻ظ´
¾ø¶Ô¶¨Á¿Ó«¹âPCRÖÐ×ö±ê×¼ÇúÏßµÄDNAµÄ»ñµÃ¡£
ÒѾÓÐ10È˻ظ´
ÇëÎÊ×öʵʱ¶¨Á¿PCRǰ¼ì²âRNA»òDNAº¬Á¿µÄÒÇÆ÷½Ðʲô
ÒѾÓÐ4È˻ظ´
ÉϺ£Êв©Ê¿ºó»ù½ðÄâÈëÑ¡Ãûµ¥£¡£¡£¡
ÒѾÓÐ16È˻ظ´
ADTechnology
¾èÖú¹ó±ö (ÖøÃûдÊÖ)
±í¹ÛÒÅ´«Ñ§×¨Òµ¹©Ó¦ÉÌ
- Ó¦Öú: 12 (СѧÉú)
- ½ð±Ò: 12309.7
- É¢½ð: 1762
- ºì»¨: 1
- Ìû×Ó: 2639
- ÔÚÏß: 50.3Сʱ
- ³æºÅ: 1787705
- ×¢²á: 2012-04-30
- רҵ: ÈËÀàÒÅ´«Ñ§
¡¾´ð°¸¡¿Ó¦Öú»ØÌû
É̼ÒÒѾÖ÷¶¯ÉùÃ÷´Ë»ØÌû¿ÉÄܺ¬ÓÐÐû´«ÄÚÈÝ|
ÀÏʦ£¬ÄúºÃ£¡¿ÉÒÔ¿´Ò»Ï£¬Õâ¸ö²úÆ·µÄÇé¿ö£¬ÊÇ·ûºÏÄúµÄʵÑéÐèÇóµÄ¡£¼Û¸ñÒªÄúʹÓÃÄÄÖÖ¹æ¸ñµÄ¡£¾ßÌåÎÊÌâ¿ÉÒÔ¼Ó¼¼ÊõQ1951545998£¡ DNA¼×»ù»¯ÃâÒß¹²³ÁµíÊÔ¼ÁºÐ »õºÅ£ºA-P-2019 EpiQuik Methylated DNA Immunoprecipitation Kit ±³¾°×ÊÁÏ£ºEpiQuik¼×»ù»¯DNAÃâÒß¹²³ÁµíÊÔ¼ÁºÐÔËÓñ¾¹«Ë¾µÄרÀû¼¼Êõ£¬ÌṩÁËÒ»ÕûÌ×±ØÐèµÄÊÔ¼ÁºÍ·½·¨À´´Óϸ°ûÖи»¼¯¼×»ù»¯DNA¡£ÊÔ¼ÁºÐÓü׻ù»¯°ûà×वÄÌØÒìÐÔ¿¹ÌåÓë¼×»ù»¯»ùÒò×éDNA(meDIP)½øÐÐÃâÒß¹²³Áµí¡£¸»¼¯ËùµÃµ½µÄ¼×»ù»¯Æ¬¶Î¿ÉÒÔ½øÐбê×¼µÄDNA·ÖÎö¡£Õû¸ö¸»¼¯¹ý³Ì»¨·Ñ²»µ½3Сʱ£¬»ñµÃ±ÈÆäËüÊÔ¼ÁºÐ¸üºÃµÄ¸»¼¯Ð§¹û¡£±¾ÊÔ¼ÁºÐ¸»¼¯µÄ¼×»ù»¯DNA¿ÉÓÃÀ´½øÐж¨ÐԺͶ¨Á¿PCR¡¢southern Ó¡¼£ÒÔ¼°DNA΢ÕóÁзÖÎö¡£ ²úÆ·ÃèÊö£ºEpiQuik¼×»ù»¯DNAÃâÒß¹²³Áµí(MeDIP)ÊÔ¼ÁºÐÖк¬ÓÐÃâÒß¹²³Áµí²¸È鶯Îïϸ°ûÖм׻ù»¯DNAËùÐèµÄËùÓÐÊÔ¼Á¡£²¢ÇÒ£¬±¾ÊÔ¼ÁºÐÖл¹ÓÐÒ»ÖÖCHIP-¼¶ 5-¼×»ù»¯¿¹Ì壬ÒÔ¼°Õý³£Ð¡ÊóµÄIgGÒõÐÔ¶ÔÕÕ¡£´Óϸ°ûÖÐÌáÈ¡³öDNA£¬´ò¶ÏÖ®ºó£¬¼ÓÈë΢¿×°åÖУ¬±»¿¹Ìå²¶»ñ¡£È»ºóDNA´Ó¸´ºÏÎïÉϽâÀëÏÂÀ´£¬Í¨¹ý±¾¹«Ë¾ÌرðÉè¼ÆµÄ¸ßËÙÀëÐÄÖù×Ó´¿»¯¡£Ï´ÍÑÏÂÀ´µÄDNA¿ÉÒÔ±»ÓÃÓÚËæºóµÄ¸÷ÖÖÀ©Ôö·´Ó¦¡£¸Ã¼×»ù»¯DNAÃâÒß¹²³ÁµíÊÔ¼ÁºÐÄÚÖ÷Òª²úÆ·×é·Ý°üÀ¨£ºCP1 (wash buffer) CP2 (antibody buffer) CP3A (pre-lysis buffer) CP3B (lysis buffer) CP4 (CHIP dilution buffer) CP5 (DNA release buffer) CP6 (reverse buffer) CP7 (binding buffer) CP8 (elution buffer) Normal mouse IgG (1 mg/ml) Anti-5-methylcytosine (1mg/ml) Proteinase K (10 mg/ml) 8-well assay strips (with frame) 8-well strip caps F-spin column F-collection tube Óû§Ö¸ÄÏÊÖ²á ²úÆ·ÌØµã£º 1¡¤Ö±½ÓÓëϸ°ûÁѽâÎïÖеÄDNA¼×»ù»¯Æ¬¶Î½øÐÐÃâÒß¹²³Áµí£» 2¡¤¸ßЧµØÅ¨Ëõ¸»¼¯¼×»ù»¯DNA£¬>95%£» 3¡¤Í¬Àà²úÆ·ÖÐ×î¿ìËÙµÄÊÔ¼ÁºÐ£¬3СʱÄÚÍê³É·´Ó¦£» 4¡¤96¿×°åģʽʹÑо¿ÈËÔ±Äܸù¾Ý×Ô¼ºÐèҪѡÔñÊÖ¹¤»òÊǸßͨÁ¿·ÖÎö£» 5¡¤¸½ÓÐDNA´¿»¯Öù×Ó£º½Úʡʱ¼äºÍÈËÁ¦£» 6¡¤²Ù×÷¼ò±ã¡¢½á¹û¿É¿¿¡¢Í³Ò»µÄ·ÖÎöÌõ¼þ£» ±£´æ½¨Ò飺ÔÚ½ÓÊÕµ½°¬µÂ¼Ä³öµÄÊÔ¼ÁºÐºóÇë°´ÕÕ˵Ã÷Ê齨Ò飬ʹÓò»Í¬µÄ±£´æÌõ¼þ»òζÈÀ´±£´æÊÔ¼ÁºÐÄÚ×é·Ö ¸ü¶àÁ´½Ó£ºhttp://www.aderr.com/cn/main.php ... 07&id=21487 |
3Â¥2014-07-11 14:16:51
bio-li
½ð³æ (СÓÐÃûÆø)
- Ó¦Öú: 1 (Ó×¶ùÔ°)
- ½ð±Ò: 973.8
- Ìû×Ó: 130
- ÔÚÏß: 239.6Сʱ
- ³æºÅ: 2432995
- ×¢²á: 2013-04-23
- רҵ: »ùÒò×éѧ

2Â¥2014-07-07 09:30:23
061719
гæ (СÓÐÃûÆø)
- Ó¦Öú: 0 (Ó×¶ùÔ°)
- ½ð±Ò: 7.9
- Ìû×Ó: 78
- ÔÚÏß: 16Сʱ
- ³æºÅ: 2990213
- ×¢²á: 2014-02-24
- רҵ: »ùÒò±í´ïµ÷¿ØÓë±í¹ÛÒÅ´«Ñ§
4Â¥2014-08-18 20:38:57
À±µÄ½·
гæ (³õÈëÎÄ̳)
- Ó¦Öú: 0 (Ó×¶ùÔ°)
- ½ð±Ò: 236.9
- Ìû×Ó: 26
- ÔÚÏß: 28.7Сʱ
- ³æºÅ: 2954782
- ×¢²á: 2014-02-04
- רҵ: ÈÑÉï¼°ÈÑÉïÏà¹ØÐÔ¼²²¡
5Â¥2022-02-10 19:58:56













»Ø¸´´ËÂ¥
50