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旭旭大将军

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[求助] 怎样在NCBI上查找基因的cDNA序列已有5人参与

英文原文节选：
1.VvDXSgene was split into 10 exons and 9 introns, and this structure corresponded to the gene prediction LOC 100249323 of V. vinifera PN40024.

2.VvDXSgene structure consists of ten exons and nine introns spread for a total of 4790 bp corresponding to the gene prediction LOC 100249323 on V. vinifera PN40024. A coding region of 2151 bp is predicted to encode for a DXS protein of 716 amino acids.

3.Gene-specific primers were designed and synthesized based on the genomic sequence of V. vinifera PN40024 deposited in the NCBI genome chromosome database under accession number NC_012011. A total of 4790 bp of theVvDXSlocus, from the initial ATG start codon to the TAG stop codon, was resequenced corresponding to base pair numbers 3759954-3764743 of NC_012011.

基因名字是VvDXS，我想找的是它的ORF-cDNA的全长，用来设计引物，但是按照原文介绍，怎么也找不到，请指教。

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Forlornhope

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【答案】应助回帖

基因序列：
http://www.ncbi.nlm.nih.gov/nuccore/NC_012011.3?from=3851155&to=3856272&report=genbank&strand=true
看到
“CDS          join(201..335,636..764,846..1010,1120..1270,1751..2022,
                  2119..2392,2712..3019,4065..4346,4424..4708,4841..4990)”
4990-201+1=4790 与GENOMIC ATG-TAG 4790相符
注意：标注的是ATG-TAG之间的序列

里面找到XM_002277883.2，点击进入找到mRNA序列（即cDNA全长序列）
http://www.ncbi.nlm.nih.gov/nuccore/359477447
里面看到“  CDS          201..2351”，2351-201+1=2151 与“Full ORF cDNA 2151bp”相符

希望楼主已经明白