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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
RXMCDM: 金币+1 2014-04-29 14:49:25
linyu042: 回帖置顶 2014-05-09 22:37:27
linyu042: 金币+20, 翻译EPI+1, ★★★很有帮助, 很受用，谢谢 2014-05-09 22:38:58
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Abstract: To screen out the most suitable medium for the subculture multiplication of cluster buds of Dendrobium officinale, we studied the influences of such factors as NAA, 6-BA, as well as organic additives banana and potato on the growth status of Dendrobium officinale by using 1/2MS as the basic medium. The results showed that the best medium for the germination of seeds is 1/2MS + NAA 0.1 mg/L + Pt 15% + sugar 30 g/L + agar 3.6 g/L; the best medium for the growth of F1 generation seedlings is 1/2MS + NAA 0.5 mg/L + potato juice 20% + sugar 20 g/L + agar 3.6 g/L; the best medium for the proliferation and strengthening of F2 generation seedlings is 1/2MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L + potato juice 20% + sugar 20 g/L + agar 3.6 g/L (the proliferation rate is up to 5.31%); the best medium for the proliferation and strengthening of F3 generation seedlings is 1/2MS + NAA 1.5 mg/L + potato juice 20% + sugar 20 g/L + agar 3.6 g/L; the best medium for the rooting is 1/2MS + NAA 1.5 mg/L + mashed potato 10% + sugar 20 g/L + agar 3.6 g/L (the rooting rate is up to 100%). |
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