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yanzihuaxue

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[求助] 内切酶

nicking endonucleases 用专业术语翻译成什么,购买时价钱如何
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gyesang

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4楼: Originally posted by yanzihuaxue at 2013-12-04 20:13:57
再麻烦下,这种酶有什么特点,就是简单的介绍...

就是切割双链的一条链,所以形成的是切刻而不是双链断裂的链

Nicking enzyme
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A nicking enzyme (or nicking endonuclease) is an enzyme that cuts one strand of a double-stranded DNA at a specific recognition nucleotide sequences known as a restriction site. Such enzymes hydrolyse (cut) only one strand of the DNA duplex, to produce DNA molecules that are “nicked”, rather than cleaved.[1][2]

They can be used for strand-displacement amplification,[3] Nicking Enzyme Amplification Reaction, exonucleotyic degradation, the creation of small gaps,[4] or nick translation.[5] The latter process has been successfully used to incorporate both radioactively labelled nucleotides and fluorescent nucleotides allowing specific regions on a double stranded DNA to be studied.[5][6] Over 200 nicking enzymes have been studied, and 13 of these are available commercially[7] and are routinely used for research and in commercial products.
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6楼2013-12-05 00:47:06
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gyesang

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★ ★
感谢参与,应助指数 +1
kx444555: 金币+2, 鼓励交流 2013-12-04 18:49:02
叫 切刻内切酶
    Nb.BbvCI
    Nb.BsmI
    Nb.BsrDI
    Nb.BtsI
    Nt.AlwI
    Nt.BbvCI
    Nt.BsmAI
    Nt.BspQI
    Nt.BstNBI
    Nt.CviPII

不同的酶价钱不一样,几大酶公司你都可以询问下
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2楼2013-12-04 18:26:56
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yanzihuaxue

铜虫 (小有名气)

引用回帖:
2楼: Originally posted by gyesang at 2013-12-04 18:26:56
叫 切刻内切酶
    Nb.BbvCI
    Nb.BsmI
    Nb.BsrDI
    Nb.BtsI
    Nt.AlwI
    Nt.BbvCI
    Nt.BsmAI
    Nt.BspQI
    Nt.BstNBI
    Nt.CviPII

不同的酶价钱不一样,几大酶公司你都可以询问下

很是感谢
3楼2013-12-04 19:47:50
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yanzihuaxue

铜虫 (小有名气)

引用回帖:
2楼: Originally posted by gyesang at 2013-12-04 18:26:56
叫 切刻内切酶
    Nb.BbvCI
    Nb.BsmI
    Nb.BsrDI
    Nb.BtsI
    Nt.AlwI
    Nt.BbvCI
    Nt.BsmAI
    Nt.BspQI
    Nt.BstNBI
    Nt.CviPII

不同的酶价钱不一样,几大酶公司你都可以询问下

再麻烦下,这种酶有什么特点,就是简单的介绍
4楼2013-12-04 20:13:57
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