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王艳冰

新虫 (小有名气)

[求助] 基因序列

P21和荧光素酶报告基因的序列分别是什么?急
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zh10246

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amisking: 回帖置顶 2013-11-27 18:01:20
王艳冰(amisking代发): 金币+5, good 2013-11-27 18:01:28
王艳冰: 金币+5, ★★★★★最佳答案 2013-11-28 08:52:56
P.pyralis (firefly) luciferase gene, complete cds
1 ctgcagaaat aactaggtac taagcccgtt tgtgaaaagt ggccaaaccc ataaatttgg
       61 caattacaat aaagaagcta aaattgtggt caaactcaca aacattttta ttatatacat
      121 tttagtagct gatgcttata aaagcaatat ttaaatcgta aacaacaaat aaaataaaat
      181 ttaaacgatg tgattaagag ccaaaggtcc tctagaaaaa ggtatttaag caacggaatt
      241 cctttgtgtt acattcttga atgtcgctcg cagtgacatt agcattccgg tactgttggt
      301 aaaatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc tctagaggat
      361 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
      421 attgcttttg tgagtatttc tgtctgattt ctttcgagtt aacgaaatgt tcttatgttt
      481 ctttagacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc
      541 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      601 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      661 gcagttgcgc ccgcgaacga catttataat gaacgtaagc accctcgcca tcagaccaaa
      721 gggaatgacg tatttaattt ttaaggtgaa ttgctcaaca gtatgaacat ttcgcagcct
      781 accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta
      841 ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
      901 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca
      961 gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg
     1021 ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccagg
     1081 tatgtcgtat aacaagagat taagtaatgt tgctacacac attgtagaga tcctattttt
     1141 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
     1201 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
     1261 tttgaagaag agctgttttt acgatccctt caggattaca aaattcaaag tgcgttgcta
     1321 gtaccaaccc tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct
     1381 aatttacacg aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt
     1441 gcaaaacggt gagttaagcg cattgctagt atttcaaggc tctaaaacgg cgcgtagctt
     1501 ccatcttcca gggatacgac aaggatatgg gctcactgag actacatcag ctattctgat
     1561 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     1621 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcagagag gcgaattatg
     1681 tgtcagagga cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     1741 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     1801 cttcttcata gttgaccgct tgaagtcttt aattaaatac aaaggatatc aggtaatgaa
     1861 gatttttaca tgcacacacg ctacaatacc tgtaggtggc ccccgctgaa ttggaatcga
     1921 tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg
     1981 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
     2041 agatcgtgga ttacgtcgcc agtaaatgaa ttcgttttac gttactcgta ctacaattct
     2101 tttcataggt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     2161 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     2221 ggccaagaag ggcggaaagt ccaaattgta aaatgtaact gtattcagcg atgacgaaat
     2281 tcttagctat tgtaatatta tatgcaaatt gatgaatggt aattttgtaa ttgtgggtca
     2341 ctgtactatt ttaacgaata ataaaatcag gtataggtaa ctaaaaa
Human p21 (WAF1) gene, partial promoter sequence (need to confirm)
1 ggatccctgt agagatgctc aggctgctga ggagggcgcg gtgcttggtc tctatgaata
       61 cgatgaccta aagcaaaaaa agaagatggc tatgtcggtg aagctctatg gaactgggga
      121 tcaggaggcc tggcagaaag gagtcctgtt tgcttctggg cagaacttgg catgatggag
      181 acgccagcca gcgagatgat gccaaccaga tttgccgaaa ttattgagaa gaatctcaaa
      241 agccgctagt agtaaaccga gtttcatatc agacccaggt cttggattga ggaacaggca
      301 atgggatcat tcctcagtgt ggccaaagga tctgacgagc cctcagtctt cttggaaatt
      361 cactacatag gcagccccaa tgcagacaaa ccaccccttg tttgttggga aaggaattac
      421 ctttgacagt ggtggtatct ccatcaaggc ttctgcaaat atggacctca tgagggccga
      481 catgggagga gctacaacta tatgctcagc cattgtgtct gctgcaaatc tcagtttgcc
      541 cattaatatt ataggtctgg cccctctgtg aaaacatgcc cagcggcaag gccaacaagc
      601 tggggatgtt gttagagcca ggaacaggaa gaccatccag gttggtaaca ctgatgctga
      661 ggggaggctc atactggctg atgcgctctg ttacgtgcac acatttaacc cgaaggtcat
      721 cctcaatgcc accaccttaa caggtgtcat agatgtagct ttggggtcag gtgccactgg
      781 ggtctttacc aattcatcct ggctctggaa caagctcttc gaggccagca ttgaaacagg
      841 ggaccgtgtc tggaggatgc ctctcttcaa acattgtaca agacaggttg tagattgcca
      901 gctggctgat gttaacaaca ttggaaaata tagatctgcg ggagcatgta catctgcggc
      961 attcctgaaa gaattcgtga ctcatcctaa gtgggcacat ttagacatag caggtgtgat
     1021 gaccaacaaa gatgaggttc cctatctatg gaaaggcatg accgggaggc ccacaaggac
     1081 tctcatagag ttcttacttc gtttcagtca agacaatgct tagttcagat actcaaaaaa
     1141 tgtcttcact ctgtcttaaa ttggacagtt gaagttaaaa ggtttttgaa tgaatggatg
     1201 aaaatatttt aaaggaggca atttatattt aaaaatgtag aacacaatga aatttttatg
     1261 ccttgatttt tttttcattt tacacaaaga tttatatatt ttttttttga gacggagtct
     1321 cactctgtca cccaggctgg agtgcaggtg gcatgatctc agctcactgc aacctccgcc
     1381 tcctaggttc aagcgattct cccacctcag ccacctgaat acctgggact acaggtgccc
     1441 accaccatgc ccggctgatt tttgtatttt taatggagac ggggtttcac catattggcc
     1501 aggctggtct caaaactcct gaccctgtga tctgcccgcc tcggcctccc aaagtgctgg
     1561 gattacaggc gtaaaccacc acgcccggcc agtatatatt tttaattgag aagcaaaatt
     1621 gtacttcaga tttgtgatgc taggaacatg agcaaactga aaattactaa ccacttgtca
     1681 gaaacaataa atccaacttt ttgtgcaaaa aaaaaaaata caaatattag ctgggcatgg
     1741 tggtgcatgc ctgtaatccc agctactcgg gaggctgagg cagaattgct tgaacctggg
     1801 aggcggagac tgcagtgagc tgagattgtg ccactgctga ctttgtctca aaaaacaaaa
     1861 caaaacaaaa aaacaaaatg aaaacaaaaa gccagggctg cctctgctca ataatgttct
     1921 atctttgttc cgcctcttct ctggggtctc acttcttggg agcctgtgtg aaggtgaatt
     1981 cctctgaaag ctgactgccc ctatttggga ctccccagtc tctttctgag aaatggtgac
     2041 attgttccca gcacttcctc tcccttccta ggcagcttct gcagccacca ctgagccttc
     2101 ctcacatcct ccttcttcag gcttgggctt tccacctttc accattcccc taccccatgc
     2161 tgctccaccg cactctgggg agggggctgg actgggcact cttgtccccc aggctgagcc
     2221 tccctccatc cctatgctgc ctgcttccca ggaacatgct tgggcagcag gctgtggctc
     2281 tgattggctt tctggccatc aggaacatgt cccaacatgt tgagctctgg catagaagag
     2341 gctggtggct attttgtcct tgggctgcct gttttcaggg aggaagggga tggtaggaga
     2401 caggagacct ctaaagaccc caggtaaacc ttagcctctt actctgaaca gggtatgtga
     2461 tctgccagca ggatccttgc gacagggctg ggatctgatg catgtgtgct tgtgtgagtg
     2521 tgtgctggga gtcagattct gtgtgtgact tttaacagcc tgctcccttg ccttcttcag
     2581 ggcagaagtc ctcccttaga gtgtgtctgg gtacacattc aagtgcatgg ttgcaaactt
     2641 ttttttttaa agcactgaat agtactagac acttagtagg tacttaagaa atattgaatg
     2701 tcgtggtggt ggtgagctag aagttataaa aaaaattctt tcccaaaaac aacaacaaaa
     2761 agaattattt cattgtgaag ctcagtacca caaaaattca ttacaataat tcattacaag
     2821 cctttattaa aaaaaatttt ctccccaaag taaacagaca gacaatgtct agtctatttg
     2881 aaatgcctga aagcagaggg gcttcaaggc agtgggagaa ggtgcctgtc ctctgctgga
     2941 catttgacaa ccagcccttt ggatggtttg tatgtatagg agcgaaggtg cagacagcag
     3001 tggggcttag agtggggtcc tgaggctgtg ctgtggccct tctggggttt agccacaatc
     3061 ctggcctgac tccagggcga ggcaggccaa gggggtctgc tgctgtgtcc tcccacccct
     3121 acctgggctc ccatccccac agcagaggag aaagaagcct gtcctccccg aggtcagctg
     3181 cgttagagga agaagactgg gcatgtctgg gcagagattt ccagactctg agcagcctga
     3241 gatgtcagta attgtagctg ctccaagcct gggttctgtt tttcagtggg atttctgttc
     3301 agatgaacaa tccatcctct gcaatttttt aaaagcaaaa ctgcaaatgt ttcaggcaca
     3361 gaaaggaggc aaaggtgaag tccaggggag gtcaggggtg tgaggtagat gggagcggat
     3421 agacacatca ctcatttctg tgtctgtcag aagaaccagt agacacttcc agaattgtcc
     3481 tttatttatg tcatctccat aaaccatctg caaatgaggg ttatttggca tttttgtcat
     3541 tttggaacca cagaaataaa ggatgacaag cagagagccc cgggcaggag gcaaaagtcc
     3601 tgtgttccaa ctatagtcat ttctttgctg catgatctga gttaggtcac cagacttctc
     3661 tgagccccag tttccccagc agtgtatacg ggctatgtgg ggagtattca ggagacagac
     3721 aactcactcg tcaaatcctc cccttcctgg ccaacaaagc tgctgcaacc acaggggttt
     3781 cttctgttca ggtgagtgta gggtgtaggg agattggttc aatgtccaat tcttctgttt
     3841 ccctggagat caggttgccc ttttttggta gtctctccaa ttccctcctt cccggaagca
     3901 tgtgacaatc aacaactttg tatacttaag ttcagtggac ctcaatttcc tcatctgtga
     3961 aataaacggg actgaaaaat cattctggcc tcaagatgct ttgttggggt gtctaggtgc
     4021 tccaggtgct tctgggagag gtgacctagt gagggatcag tgggaataga ggtgatattg
     4081 tggggctttt ctggaaattg cagagaggtg catcgttttt ataatttatg aatttttatg
     4141 tattaatgtc atcctcctga tcttttcagc tgcattgggt aaatccttgc ctgccagagt
     4201 gggtcagcgg tgagccagaa agggggctca ttctaacagt gctgtgtcct cctggagagt
     4261 gccaactcat tctccaagta aaaaaagcca gatttgtggc tcacttcgtg gggaaatgtg
     4321 tccagcgcac caacgcaggc gagggactgg gggaggaggg aagtgccctc ctgcagcacg
     4381 cgaggttccg ggaccggctg gcctgctgga actcggccag gctcagctgc tccgcgctgg
     4441 gcagccagga gcctgggccc cggggagggc ggtcccgggc ggcgcggtgg gccgagcgcg
     4501 ggtcgcctcc ttgaggcggg cccgggcggg gcggttgtat atcagggccg cgctgagctg
     4561 cgccagctga ggtgtgagca gctgccgaag tcagttcctt gtggagccgg agctgggcgc
     4621 ggattcgccg aggcaccgag gcactcagag gaggtgagag agcggcggca gacaacaggg
     4681 gaccccgggc cggcggccca gagccgagcc aagcgtgccc gcgtgtgtcc ctgcgtgtcc
     4741 gcgaggatgc gtgttcgcgg gtgtgtgctg cgttcacagg tgtttctgcg gcaggtgaat
     4801 gacgggcgtg ggtcggtgcg cgctcggctt gcgcacacgg tgtctctaag tgcgcgggtg
     4861 acgagggtcg ggatgtgccg gagaccccgg gcggagagcg ggattacaag tacaggaatc
     4921 cctggccacg ctccccgccc ctggaaaccc agctggggcg agggagggcg tggacgggac
     4981 cgttctggga gctcgccttt ggctgcggtt ggctccaggc cccaggcgca gtttgctcgc
     5041 ggcgtgggga tgaagtccgt gtccctggag gggcccagga agggcgagga aagcggagtg
     5101 gagtaagttc gtctaggatc ggtcccgggt ggctctggga tcc
2楼2013-11-27 17:55:57
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王艳冰

新虫 (小有名气)

引用回帖:
2楼: Originally posted by zh10246 at 2013-11-27 17:55:57
P.pyralis (firefly) luciferase gene, complete cds
1 ctgcagaaat aactaggtac taagcccgtt tgtgaaaagt ggccaaaccc ataaatttgg
       61 caattacaat aaagaagcta aaattgtggt caaactcaca aacattttta ttatatacat
  ...

谢谢你,我想问一下,你这是在NCBI上查的吗
3楼2013-11-28 08:49:52
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王艳冰

新虫 (小有名气)

引用回帖:
2楼: Originally posted by zh10246 at 2013-11-27 17:55:57
P.pyralis (firefly) luciferase gene, complete cds
1 ctgcagaaat aactaggtac taagcccgtt tgtgaaaagt ggccaaaccc ataaatttgg
       61 caattacaat aaagaagcta aaattgtggt caaactcaca aacattttta ttatatacat
  ...

因为我一直在查,找到的感觉都不对,我想问一下具体的方法可以吗
4楼2013-11-28 08:51:10
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zh10246

铁杆木虫 (文坛精英)

引用回帖:
4楼: Originally posted by 王艳冰 at 2013-11-28 08:51:10
因为我一直在查,找到的感觉都不对,我想问一下具体的方法可以吗...

是啊,NCBI上,最上边偏左的地方有个下拉式菜单,选Nucleotid,然后输入
firefly luciferase,第一个就是
5楼2013-11-28 09:01:39
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王艳冰

新虫 (小有名气)

引用回帖:
5楼: Originally posted by zh10246 at 2013-11-28 09:01:39
是啊,NCBI上,最上边偏左的地方有个下拉式菜单,选Nucleotid,然后输入
firefly luciferase,第一个就是...

这个我查到了,主要是P21的序列,我的P21是2000多bp,在上面不确定是哪个
6楼2013-11-28 09:30:48
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