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1.the precipitate was dissolved in 20mM Tris-HCl buffer(PH7.4) and dialysed against the same buffer.

2.after 16h of dialysis, the sample was applied onto a ConA sepharose 4B column pre-equilibrated with equilibration buffer(20mM Tris-HCl, 1M NaCl,1mM CaCl2, MgCl2, MnCl2) at a flow rate of 0.1 ml/min.

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