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[求助]
制备的金纳米棒如何确定浓度?
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按照文献 制备金纳米棒：Chem. Mater. 2005, 17, 4636-4641 步骤如下：Gold Nanorod Synthesis. Gold nanorods were prepared exactly as described by Sau et al.,33 but scaled up to increase the quantity. Gold seed particles were prepared by adding 250 íL of 10 mM HAuCl4a3H2O to 7.5 mL of 100 mM cetyltrimethylammonium bromide (CTAB) with brief, gentle mixing. Next, 600 íL of freshly prepared, ice-cold 10 mM NaBH4 solution was added followed by mixing for 2 min. The pale brown seed solution was stable and usable for several days. The nanorod growth solution was prepared by adding the following reagents to a plastic tube in the following order and then gently mixing: 40 mL of 100 mM CTAB, 1.7 mL of 10 mM HAuCl4a3H2O, 250 íL of 10 mM AgNO3. Next, 270 íL of 100 mM ascorbic acid was added, which changed the solution from brown-yellow to colorless. To initiate nanorod growth, 420íL of the seed solution was added to the growth solution, mixed gently, and left still for 3 h. 之后离心，纯化，得到的AuNR@CTAB储存在水中备用。 接下来，文章中就用3 mL (25 nM) of the originalAuNR@CTAB in water. 我想知道的就是金纳米棒的浓度（25nM) 是如何得出的呢？离心后用多少量的水储存也没有说。 制备出来的金棒溶液怎么计算其浓度? |
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