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whisht

银虫 (小有名气)

[求助] <微生物方向> 60金币求助-审稿人的反馈意见解读及详细对策

question 1:
this manuscript has a major flaw and this is lack of true replication (only one reactor per treatment) so it cannot statistically discern whether earthworms improved or not the stabilization process.

question 2:
DNA: please put the information on legend of figure 3 (alignment, phylogenetic method… here)
Response:  

question 3:
Results and discussion
Lack of statistical tests makes this part meaningless. Authors could try to test with their phylogenetic tree some tests like UNIFRAC
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nono2009

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【答案】应助回帖

感谢参与,应助指数 +1
审稿人意见很明确:Lack of statistical tests
补充实验吧
3楼2013-05-01 13:24:46
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hongxueren

金虫 (正式写手)

【答案】应助回帖

★ ★
感谢参与,应助指数 +1
whisht(esub代发): 金币+2, 鼓励有价值的应助 2013-05-04 20:29:16
2比较容易
1、3都说明要补充实验 要有统计意义上的说服力
2楼2013-05-01 11:03:51
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whisht

银虫 (小有名气)

引用回帖:
2楼: Originally posted by hongxueren at 2013-05-01 11:03:51
2比较容易
1、3都说明要补充实验 要有统计意义上的说服力

DNA: please put the information on legend of figure 3 (alignment, phylogenetic method… here)
我在图3的说明中已经说明:Neighbor-joining phylogenetic tree of 16S rRNA gene sequences. Sequences from the present study and close relatives were aligned by multiple alignments using CLUSTALW. 还有什么没有写清楚,我实在不清楚。
能否帮我解读审稿人到底是什么意思?本人不是微生物出身的。
谢谢啦!
4楼2013-05-01 14:54:57
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whisht

银虫 (小有名气)

引用回帖:
3楼: Originally posted by nono2009 at 2013-05-01 13:24:46
审稿人意见很明确:Lack of statistical tests
补充实验吧

question 3:
Results and discussion
Lack of statistical tests makes this part meaningless. Authors could try to test with their phylogenetic tree some tests like UNIFRAC

论文中采用方法如下:
For phylogenetic analysis, sequences were aligned with Clustal X. A phylogenetic tree was constructed using MEGA 4.0, and reference sequences used in the tree construction were acquired from GenBank.
我不清楚审稿人说的“ their phylogenetic tree some tests like UNIFRAC”方法是怎样的,能否具体告知?谢谢!
5楼2013-05-01 14:59:02
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