用1389/3*110方法算最后结果约为51KD,而用114718.07/1000方法算结果约为115KD,其实我最不明白的是为什么两种算法差值会有这么大。还有一个就是做完western blot 之后跑的胶都能分别看到这两个大小的泳道,所以我就不知道我的蛋白大小到底应该是哪个。谢谢!!麻烦帮忙解答一下!
the former one is the average or raw molecular weight, the second one should be the relatively accurate molecular weight
you can also use this website to calculate the MW