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万岁子郎

木虫 (正式写手)

[求助] 求请解读审稿人提出的关于我文章中方差分析的审稿意见

各位大侠,最近投了一篇材料的文章,然后在文章中,我用one way ANOVA对我的数据进行了分析。但是审稿人说我的分析是错的,还请各位帮我看看审稿人具体是什么意思,因为我不是数学背景,所以对他提的意见,好几处不大明白!先附上审稿意见:

However, there a big issue in data analysis to prevents this manuscript to
be acceptable for publication in its present. It is a pity. I suggest the
authors follow my suggestions and redo statistical analysis, redo table and
rediscuss the data. Because your analysis is wrong, it is no useful to
review all sections. I recommend the manuscript could be acceptable after
the following issues are addressed and resubmit the paper

1. Suggestions: Redo your data analysis using a correct statistical analysis
method, provide which multi-treatment method you use, what method you use
for check data normality, which method you use to check residue and variance
assumptions, provide analysis model, indicate fixed and random effects in
your model. Provide SEM data in your table. Provide p value in your table.
Provide n= ?? in your table that you use for statistical analysis


2. In the manuscript, you said you used Fisher test to separate mean.
Fishers protected LSD method (based on T test) has very high error I. You
set error 5% but actually error is 21%. Too high. See the following Table.


Table 1. The probability of type I error using t test for multi-sample pair
comparison

K
(how many means:  )
How many treatments T test: α significant level

0.20 0.10 α =0.05 0.02 α =0.01 0.001

2 0.20 0.10 0.05 0.02 0.01 0.001
3 0.41 0.23 0.13 0.05 0.03 0.003
4 0.58 0.36 0.21 (21%) 0.09 0.05 0.006
5 0.71 0.47 0.29 (29%) 0.13 0.07 0.009
10 0.96 0.83 0.63 0.37 0.23 0.034
20 1.00 0.98 0.92 0.71 0.52 0.109

1.00 1.00 1.00 1.00 1.00 1.00


3. You said you studied “the effects of different levels of static
deformation on the orientation and content of different secondary structures
in single A. pernyi silk fibers.” When treatments are quantitative data, we
have to check linear, quadratic and cubic effect of treatment levels on
response parameters. You did not do that.

4. We discuss the results based on correct statistical analysis. You can see
that you analyses are incorrect. You should redo the analysis before you can
discuss. If treatment level has quadratic effect, you should give optimal
point using 2nd derivative function. If your treatment has cubic effect, you
should give max and min points.

其实在我的文章中,我只是用one way ANOVA 比较了不同拉伸条件下蛋白质结构的变化如下表(这是其中之一,其他基本差不多):

conformation                                                   content (%)
                            strain=0        strain=0.1         strain=0.2         strain=0.3
β-sheet                    38±3                38±2                38±4                37±3
random coil*           44±1             46±2                46±2                48±3
α-helix**               18±3                17±3                15±4                15±3
* It shows significant difference by one way ANOVA analysis (significance level at p < 0.0001).
** It shows significant difference by one way ANOVA analysis (significance level at p < 0.05).

请教各位,我应该如何回答审稿人啊?或者我需要修改?谢谢各位大侠帮助!在这里给各位拜早年了! 新年快乐!
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万岁子郎

木虫 (正式写手)

引用回帖:
3楼: Originally posted by yaofengluan at 2013-02-09 09:56:55
你自己搜索下correct statistical analysis吧,这个分析的前提你要先验证一下,就是第一个审稿人提的正态性,残差以及方差一致性,指明固定效应和随机效应等,样本数以及a等。T test 犯第一类错误的概率大(即弃真) ...

非常感谢!祝福新年快乐!!!
5楼2013-02-09 16:13:09
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onesupeng

金虫 (职业作家)

【答案】应助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感谢参与,应助指数 +1
万岁子郎: 金币+5, 有帮助 2013-02-09 16:13:34
万岁子郎: 金币+5, 有帮助 2013-02-09 16:13:51
万岁子郎: 金币+5, 有帮助 2013-02-09 16:15:59
如果你觉得杂志很不错,继续认真修改。如果你觉得是鸡勒,就换个杂志。说实话这样做和重写一篇文章差不多
长期招收博士生,参见http://fsl-unsw.com
2楼2013-02-09 09:10:35
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yaofengluan

至尊木虫 (知名作家)

你自己搜索下correct statistical analysis吧,这个分析的前提你要先验证一下,就是第一个审稿人提的正态性,残差以及方差一致性,指明固定效应和随机效应等,样本数以及a等。T test 犯第一类错误的概率大(即弃真),要换一种多重比较的方法。我也不是很清楚,这些供你参考。
我个人认为既然两个审稿人都提到了,你就要认真对待,与杂志水平无关。这个应该还是比较容易解决的,请不要灰心。
3楼2013-02-09 09:56:55
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nono2009

超级版主 (文学泰斗)

No gains, no pains.

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【答案】应助回帖

★ ★ ★ ★ ★
感谢参与,应助指数 +1
万岁子郎: 金币+5 2013-02-09 16:16:23
审稿人极其认真、专业、而且nice的,按其要求去做,被录用的可能性应该很高的。
4楼2013-02-09 10:16:41
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