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万岁子郎木虫 (正式写手)
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[求助]
求请解读审稿人提出的关于我文章中方差分析的审稿意见
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各位大侠，最近投了一篇材料的文章，然后在文章中，我用one way ANOVA对我的数据进行了分析。但是审稿人说我的分析是错的，还请各位帮我看看审稿人具体是什么意思，因为我不是数学背景，所以对他提的意见，好几处不大明白！先附上审稿意见： However, there a big issue in data analysis to prevents this manuscript to be acceptable for publication in its present. It is a pity. I suggest the authors follow my suggestions and redo statistical analysis, redo table and rediscuss the data. Because your analysis is wrong, it is no useful to review all sections. I recommend the manuscript could be acceptable after the following issues are addressed and resubmit the paper 1. Suggestions: Redo your data analysis using a correct statistical analysis method, provide which multi-treatment method you use, what method you use for check data normality, which method you use to check residue and variance assumptions, provide analysis model, indicate fixed and random effects in your model. Provide SEM data in your table. Provide p value in your table. Provide n= ?? in your table that you use for statistical analysis 2. In the manuscript, you said you used Fisher test to separate mean. Fishers protected LSD method (based on T test) has very high error I. You set error 5% but actually error is 21%. Too high. See the following Table. Table 1. The probability of type I error using t test for multi-sample pair comparison K (how many means: ) How many treatments T test: α significant level 0.20 0.10 α =0.05 0.02 α =0.01 0.001 2 0.20 0.10 0.05 0.02 0.01 0.001 3 0.41 0.23 0.13 0.05 0.03 0.003 4 0.58 0.36 0.21 (21%) 0.09 0.05 0.006 5 0.71 0.47 0.29 (29%) 0.13 0.07 0.009 10 0.96 0.83 0.63 0.37 0.23 0.034 20 1.00 0.98 0.92 0.71 0.52 0.109 1.00 1.00 1.00 1.00 1.00 1.00 3. You said you studied “the effects of different levels of static deformation on the orientation and content of different secondary structures in single A. pernyi silk fibers.” When treatments are quantitative data, we have to check linear, quadratic and cubic effect of treatment levels on response parameters. You did not do that. 4. We discuss the results based on correct statistical analysis. You can see that you analyses are incorrect. You should redo the analysis before you can discuss. If treatment level has quadratic effect, you should give optimal point using 2nd derivative function. If your treatment has cubic effect, you should give max and min points. 其实在我的文章中，我只是用one way ANOVA 比较了不同拉伸条件下蛋白质结构的变化如下表（这是其中之一，其他基本差不多）： conformation content (%) strain=0 strain=0.1 strain=0.2 strain=0.3 β-sheet 38±3 38±2 38±4 37±3 random coil* 44±1 46±2 46±2 48±3 α-helix** 18±3 17±3 15±4 15±3 * It shows significant difference by one way ANOVA analysis (significance level at p < 0.0001). ** It shows significant difference by one way ANOVA analysis (significance level at p < 0.05). 请教各位，我应该如何回答审稿人啊？或者我需要修改？谢谢各位大侠帮助！在这里给各位拜早年了！ 新年快乐！ |
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