Cloning and identification of novel cellulase genes from uncultured microorganisms in rabbit cecum and characterization of the expressed cellulases(封毅AMB2007)
Optimal pH were tested by the enzyme assays at 37°C in buffers of different pH (citrate–phosphate buffer, pH 3.0∼7.0; 0.1 M sodium–phosphate buffer, pH 6.0∼8.0;0.1 M Tris–HCl buffer, pH 7.0∼9.0; 0.1 M glycine–NaOH buffer, pH 8.6∼10.0).
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