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xgxofdream银虫 (著名写手)
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[求助]
AFM能用来做溶液样本中的纳米颗粒的表征吗
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| 大家好，我合成的纳米颗粒不稳定，大慨有20min的稳定期。因此我想避免复杂的制样过程，而去直接表征样本溶液中的纳米颗粒。听说AFM可以直接表征溶液样本，是吗？谁有相关的经验可以告诉我下吗？ |
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xgxofdream
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这是原始文献中用来表征这种颗粒的AFM方法： The sample of dsDNA or ssDNA (10 μl) was deposited onto a freshly cleaved mica surface (Ted Pella, Inc.) and left to adsorb for 10 min. Then 10 mM MOPS (12.5 mM Mg2+) (500 μl) was added to the liquid cell and the sample was scanned in a tapping mode using Agilent AFM series 5500 (Agilent Technologies, USA) with 0.08 N/m force constant cantilever of a Silicon nitride cantilevers with sharpened Pyramidal tip (OMCL-TR400PSA, Olympus, Atomic Force F&E GmbH, Mannheim, Germany). After engagement the tapping amplitude set point was typically 2.0 volts and the scan rates ranged from 1-2 Hz. During the liquid AFM imaging the better imaging was in most cases obtained with minimal loading forces applied and optimized feedback parameters. Several AFM images, all 512 × 512 pixels, were obtained from separate locations across the mica surfaces to ensure reproducibility of the results. All the images were analyzed by using Gwyddion 2.16 software. 我看了，貌似没什么制样过程。你们帮我研究一下，难度在哪里？我打算请公司或者高校的人帮我做。 |
12楼2012-08-09 15:37:22
mlanqiang
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2楼2012-08-09 06:01:28
zygoal
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3楼2012-08-09 10:04:10
xgxofdream
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4楼2012-08-09 10:31:28