至尊木虫 (知名作家)
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【答案】应助回帖
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yanruo: 金币+15, 翻译EPI+1, ★★★★★最佳答案, 非常感谢您！ 2012-08-08 20:50:42
3.1Induction of apoptosis
Apoptosis, including an autophagy, necrosis, senescence and cell death in one way. Compounds able to make a human non-small cell lung cancer cell lines (PLA-801) apoptosis undergo. Apoptosis is activating an endogenous inhibition of enzymes, the endogenous inhibitor enzymes cut the DNA strand, DNA is degradatied into small fragment between 180 ~ 200 bp, thereby apoptosis enable happened. Montririttigri and others from studies have shown that compounds extracted from Stephania venosa (Blume), an ethanol extract of ovarian cancer cell line (Skov3) cytotoxicity. A Park Philippine class of compounds to DNA degradation ovarian cancer cell lines by MTT assay proved.
3.2 Cancer cell proliferation is reduced or inhibited
By detecting the mitotic index and cell cycle analysis of cell proliferation. The cell cycle dependent cyclin activation and cyclin-dependent kinase (of CDKs). Its function is to start in the G1 phase to S phase, and the split into the G2 / M phase. Compounds extracted from Stephania venosa and its ethanol extract addition to make ovarian cancer cell apoptosis, they proliferation of tumor cells are significantly inhibited. Compound 2 malignant gliomas, the mechanism of action is to inhibit cancer cell proliferation. Daneli with flow cytometry showed that the compound treatment of malignant glioma cell lines (U138-MG) for 24 h, the percentage of G2 / M phase cells increased significantly, indicating that the compound 2 by the cell cycle resistance off in the G2 / M phase to reduce the spread of cancer to achieve anti-cancer effect. Compound 3 with tumor cell lines co-cultured cell lines 5h of cell cycle arrest at G2 / M phase, and then arrest in G1 phase cell cycle arrest in S phase of the cell lines is the complete cessation of DNA replication.
3.3 Inhibition of DNA topoisomerase
DNA topoisomerase is present in the nucleus of a class of enzymes capable of catalyzing DNA strand breaks and combined to control the state of DNA topology. Alkaloids unique nucleus structure determines the molecules of these compounds show a relatively flat structure, so that it selectively with DNA topoisomerase II, the target DNA efficient combination of the formation of molecular complexes is difficult to dissociation alkaloids through this competitive binding inhibition of DNA topoisomerase II catalytic activity and show cytotoxicity. The anticancer mechanism of compound 4 is that it co-inserted into the DNA strand through and topoisomerase competition to win the topoisomerase target DNA and inhibits its activity, so as to achieve the anti-cancer effect." |
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