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火舞精灵3

铜虫 (正式写手)

[求助] 悬赏50金币!!!求助!翻译一段反相色谱与质谱英文操作步骤

非常急!!!!悬赏50金币!!求助高手!!
Peptides were separated on a 20-cm column with a 75 µm inner diameter packed with Dr. Maisch GmbH reversed-phase material Reprosil-Pur 120 C18-AQ, 1.9 µm resin (SinoAmerican Proteomics LLC).  Separation was performed using 220-min runs on a Waters NanoAcquity liquid chromatography with a binary buffer system of 2% acetonitrile in 0.1% formic acid (buffer A) and 99.9% acetonitrile in 0.1% formic acid (buffer B) interfaced to a LTQ-Orbitrap Velos (Thermo Fisher Scientific).  The sample was loaded onto the trap column at a flow rate of 10 µl/min for 1 min and then separated at a flow rate of 300 nl per min by an initial linear gradient of buffer B from 6% to 26% up to 180 min followed by a 26% to 80% buffer B gradient for 40 min.  The mass spectrometer was operated in data-dependent mode, with one full scan acquisition in the Orbitrap at 30,000 resolution (at 400 m/z) with an automatic gain  control (AGC) target value of 10^6 ions.  The 10 most intense ions with a charge >1 were selected for tandem data-dependent analysis using a hybrid method of alternating collision-induced dissociation and higher energy collision dissociation fragmentation techniques (CID and HCD, respectively)1. CID scans were collected at an AGC target value of 5,000 with a maximum injection time of 25 ms.  HCD scans were collected at 7,500 resolution with an AGC target value of 30,000 and a maximum injection time of 150 ms.  Dynamic exclusion was set to +/- 0.005 m/z for 60 seconds with an early expiration count of 1 and signal to noise of 2.[ 来自科研家族 生化掘金 ]
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zhoumin235

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火舞精灵3: 金币+30, ★★★很有帮助 2012-05-31 17:01:37
我是做液相的 所以只能翻译液相部分 质谱部分翻译不好  呵呵  如果翻译有问题敬请指正

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