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mike_308

银虫 (小有名气)

[求助] 求助用罗丹明B异硫氰酸酯(36877-69-7)标记牛血清蛋白的详细方法

如题~用罗丹明B异硫氰酸酯标记牛血清蛋白是否和用FITC是一样的?
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mike_308

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9楼: Originally posted by xhjggl at 2012-04-29 19:37:45:
产品编号是786-058,而不是36877-69-7,无语!!!!

36877-69-7是罗丹明B异硫氰酸酯的CAS号
产品编号786-058的试剂不是罗丹明B异硫氰酸酯
因为我是写材料所以,需要找到一份有出处的关于是罗丹明B异硫氰酸酯的使用说明书
10楼2012-04-30 11:57:14
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xhjggl

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【答案】应助回帖

★ ★ ★
感谢参与,应助指数 +1
zhaohq1209: 金币+3, 鼓励应助~欢迎交流~thanks 2012-04-29 09:44:26
HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine)
蛋白罗丹明标记试剂盒       
Description:
(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.(5/6) TAMRA absorbs green visible light at 546nm and emits an orange-red visible light at a maximum emission of 575nm.The NHS ester group provides the simplest and most commonly used group for labeling proteins. The succinimidyl ester group reacts with primary amines in lysine side chains and N-terminal amines forming a stable, covalent amide bond.This kit utilizes Tube-O-REACTOR™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort. The (5/6) TAMRA-SE labeling reagent is also available in our single use OneQuant™ format.
Features:
Dye preweighed and supplied in single use  vials (Also available separately);Suitable for most proteins;Utilizes  single Tube Reactor for minimum hands on time and no protein loss.application for Labeling of proteins, peptides and nucleic acids with a red fluorescent dye.;Suitable for antibody labeling.Store: -15~-20℃
编号        包装        价格        产地
786-058        5 Reactions        2,709        GBios
2楼2012-04-17 13:57:40
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mike_308

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2楼: Originally posted by xhjggl at 2012-04-17 13:57:40:
HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine)
蛋白罗丹明标记试剂盒       
Description:
(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on te ...

有详细一些的使用说明吗?
3楼2012-04-20 12:11:10
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xhjggl

木虫 (正式写手)

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有,可以到上海生工网站上下载说明书看看

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4楼2012-04-20 13:22:11
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