reviewer#1:The manuscript has been very well drafted and its results are presented well. The concept of the study is well articulated and can be understood with a lot of easy. However the following remarks may boost the work being presented.
ⅰ) consider avoiding to start with numbers in sentences e.g 2 ml.
Answer: thank you very much for the suggestion, we have revised the whole paper and made 7 amendments in "Materials and Methods" section (page 3, line 16; page 4, line 20; page 5, line 18; page 5, line 21; page 6, line 2; page 6, line 3; page 6, line 4.).
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