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love36183618木虫 (初入文坛)
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[求助]
请大家帮忙翻译一下，非常感谢！
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DNA对BCDO荧光光谱的影响 在上述较佳实验条件下，扫描BCDO与小牛胸腺DNA浓度在0.0、1.0、2.0、3.0、4.0、5.0、6.0 (浓度单位为mg•L-1，数值从小到大，即由a→g)范围内7个不同浓度对BCDO作用的荧光光谱，结果如图3.30所示。 由图3.30可知，随着DNA浓度的增加，BCDO的在λEm＝517.8nm处的荧光发射光谱的峰位置保持不变，但是荧光强度逐渐减弱，即荧光猝灭程度越来越强。该体系中DNA加入引起BCDO的荧光强度降低的现象和已经知道的一些荧光分子在DNA存在时荧光强度降低的现象是一致的，通常情况下认为其作用机理是：当某些荧光小分子嵌插到DNA的碱基对中时，该荧光物质的激发态与鸟苷G离得较近，由于鸟苷G本身有非常强的给电子能力，使得该物质与DNA的碱基之间发生电子的转移。而本组自制的BCDO的共扼平面部分很可能嵌插到了DNA双螺旋内部的碱基对之间，使得BCDO与鸟苷G之间发生电子的转移，导致无能量转移，导致荧光猝灭。 |
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Mally89: 金币+1, 感谢应助！~ 2012-04-02 20:33:43
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Mally89: 金币+1, 感谢应助！~ 2012-04-02 20:33:43
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The effects of DNA on BCDO's fluorescence spectra. Under the better experimental condition, we studied the effects of DNA on BCDO's fluorescence spectra at seven calf thymus DNA concentrations, i.e., 0.0, 1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 (mg/L), and the results are shown in Figure 3.30. From figure 3.30, we see that as the concentration of DNA increases the peak position of BCDO's fluorescence emission spectra did not change at λEm＝517.8nm, however, the fluorescence intensity decreased continuously (i.e., fluorescence quenching getting stronger). In our study system, the phenomenon that the addition of DNA decreased BCDO’s fluorescence intensity was consistent with the phenomena that fluorescence intensity decreases with the presence of DNA, the commonly accepted mechanism is that the fluorescent materials’ excited states become closer to Guanosine when certain small fluorescent molecules inserted into the base pairs of DNA, and Guanosine’s very strong electron-donating capacity makes electron transfer between the material and DNA bases. It is very likely that the conjugate plane of BCDO made by ourselves inserted into the base pairs inside of DNA double helix and makes the electron transfer between BCDO and Guanosine, and resulted in energy transfer failure and fluorescence quenching. |
2楼2012-04-01 16:28:49
