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[资源] DNA-Protein Interactions3rd ed.pdf

Contents
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
1 Filter-Binding Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Peter G. Stockley
2 Electrophoretic Mobility Shift Assays for the Analysis
of DNA–Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Manon Gaudreault, Marie-Eve Gingras, Maryse Lessard,
Steeve Leclerc, and Sylvain L. Guérin
3 DNase I Footprinting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Benoît Leblanc and Tom Moss
4 Exonuclease III Footprinting on Immobilized DNA Templates . . . . . . . . . . . . . . 49
Patrizia Spitalny and Michael Thomm
5 Hydroxyl Radical Footprinting of Protein–DNA Complexes . . . . . . . . . . . . . . . . . 57
Indu Jagannathan and Jeffrey J. Hayes
6 The Use of Diethyl Pyrocarbonate and Potassium Permanganate
as Probes for Strand Separation and Structural Distortions in DNA . . . . . . . . . . . 73
Brenda F. Kahl and Marvin R. Paule
7 Uranyl Photofootprinting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Peter E. Nielsen
8 Identification of Protein/DNA Contacts with Dimethyl
Sulfate: Methylation Protection and Methylation Interference . . . . . . . . . . . . . . . 97
Peter E. Shaw and A. Francis Stewart
9 Ethylation Interference Footprinting of DNA–Protein Complexes . . . . . . . . . . . . 105
Iain W. Manfield and Peter G. Stockley
10 Site-Directed Cleavage of DNA by Protein–Fe(II) EDTA Conjugates
Within Model Chromatin Complexes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
David R. Chafin and Jeffrey J. Hayes
11 Identification of Nucleic Acid High-Affinity Binding Sequences
of Proteins by SELEX. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
Philippe Bouvet
12 Identification of Sequence-Specific DNA-Binding Proteins
by Southwestern Blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
Simon Labbé, Jean-François Harrisson, and Carl Séguin
13 Footprinting DNA–Protein Interactions in Native Polyacrylamide
Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline–Copper. . . . . . . . . 163
Athanasios G. Papavassiliou
14 Determination of a Transcription Factor-Binding Site
by Nuclease Protection Footprinting onto Southwestern Blots . . . . . . . . . . . . . . . 201
Athanasios G. Papavassiliou
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