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[求助] 这是什么实验方法？请高人指点！

2.8. Measurement of rat platelet adenylate cyclase (AC) and guanylate
cyclase (GC)
Washed platelet after ultrasonication was centrifuged at 11,000 g for 15 min . AC was into the supernatant and the GC into precipitation. The concentrations of AC and GC were measured and adjusted to 1 mg/ml. Reaction systemof 200 µl included the following composition (mmol/L): GTP 0.2 or ATP 0.5, Tris–HCl 50 (pH 8.0), KCl 200, ATP 0.1, MgCl2 1, DTT 2, PMSF 1, GC or AC 50 µl. Reaction started
from the mixing of reaction system at 37 °C for 15 min and concluded by 10% perchloric acid of 300 µl, following the neutralization of 1.01 mol/L KOH of 500 µl and centrifugation at 825 g for 10 min. cAMPand cGMP insupernatants was were measured with a cAMP and cGMP EIA system. Activities of AC or GC were calculated by the following equation
Activity of AC= cAMP（ pmol ）/Content of AC (mg ) .15 min
Activity of GC= cGMP( pmol )/Content of GC （mg ).15 min:


这种方法是放射性同位素免疫吗？
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