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xiaokaizi

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huojinlong9287(金币+50, 翻译EPI+1): 2011-10-02 16:25:45

The results showed that the sequence of AcGFP1 gene in pET32a-AcGFP1 recombinant plasmid was consistent with AcGFP1 gene in pIRES-AcGFP1 plasmid from Clontech Ltd, proving that the prokaryotic expression vector with AcGFP1 gene of green fluorescent protein was constructed successfully. And all of the pET32a-AcGFP1 plasmids transformed in E. coli Rosetta(DE3) were high efficient expression in SDS-PAGD after induced by different concentrations of IPTG.

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