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[求助]
双向电泳提总蛋白，如何除盐啊？
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我用TCA丙酮沉淀法，提的肌肉总蛋白，电压一直上不去，想除盐，用什么方法好啊 注释：除盐步骤到了1000V,升压的时候没到4000V. |
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Perfect-FOCUS™ 2D电泳样品清除试剂 Description: Perfect-FOCUS™ is designed to clean and concentrate protein samples that give poor protein spot resolution during 2D electrophoresis. Protein samples containing interfering agents, including ionic detergents, metal ions, substrates, substrate analogs, inhibitors, and other charged molecules, routinely result in smeared or low resolution gels.Protein solutions are treated with Perfect-FOCUS™ that quantitatively precipitates the proteins, which are subsequently collected by centrifugation. The non-protein interfering agents are washed away and the protein pellet is ready to be solubilized in an appropriate sample loading buffer.The collected protein has conductivity <50µS and is substantially free from non-protein agents, which improves spot resolution and greatly reduces spot streaking.Perfect-FOCUS™ treated protein samples are compatible with mass spectrometry analysis and show identical peptide spectra.Store：18～25℃。 Features: Removes interfering agents from protein solutions.;Conductivity lower than 50µS after treatment.;Samples free from non-protein agents;Treated samples suitable for running 2D gels;the Kit suitable for 50 x 1-100µl protein solutions. suitable for concentrating and cleaning protein samples for isoelectric focusing (IEF) and 2D-gel electrophoresis applications. 编号 包装 价格 产地 786-124 50 Assays 2444 G Bios |
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