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huojinlong8610金虫 (小有名气)
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[求助]
润色 生物学
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The signal peptide prediction performed by SignalP 3.0 on the basis of a combination of several artificial neural networks and hidden Markov models revealed that BMI RBKS contained a potential signal peptide with 58.8% probability. However, we search signal peptide using human and cattle RBKS protein sequences by SignalP 3.0, there is not a singal peptide found, the probability is low 1%. The reason about difference and conflict of pig , human and cattle RBKS signal peptide need further study later. RBKS was discovered more than 50 years ago, and there has been much progress in understanding this important enzyme within the last decade. But the molecular mechanisms of which actors and how target it remain a central unresolved problem for biochemists and pharmacologists. For this reason, the homology modeling of the BMI RBKS protein was carried out using human ribokinase (2fv7 Chain: B) as the template. Our results indicate that the conserved domain (RBKS, 17-323AA) of pig RBKS protein mainly exists αβα sandwich domain and β-barrel. It suggests that these domains may play a pivotal role in RBKS activity. This first pig structural model of RBKS should prove useful reference in designing studies aimed at understanding how RBKS interacts with unknown protein partners for explaining its various functions. , |
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huojinlong8610
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4楼2011-09-01 10:25:57
hefang2005
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sltmac(金币+2): 辛苦 2011-09-01 10:44:32
sltmac(金币+15): 尽管没按楼主要求，还是给点辛苦费，欢迎常来~~ 2011-09-07 07:47:48
sltmac(金币+2): 辛苦 2011-09-01 10:44:32
sltmac(金币+15): 尽管没按楼主要求，还是给点辛苦费，欢迎常来~~ 2011-09-07 07:47:48
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SignalP 3.0 Server 是计算机辅助预测蛋白质位置的软件版本。 基于几种人工神经网络与隐藏的马克模型，利用SignalP 3.0 系统预测的信号肽显示出 BMI RBKS蛋白包含一个潜在的具有58.8%的概率存在的信号肽。然而，我们使用SignalP3.0搜寻了人类与牛RBKS 蛋白序列，没法找到任何信号肽，其概率低至1%。此不同区别的原因以及猪、人、牛的RBKS 信号肽需要在将来进一步研究。 RBKS蛋白在50年前就被发现，在最近10年对理解这种重要的酶取得了巨大进步。但是其分子机制以及如何相应信号对生物化学家与药理学家而言仍然是一个没有解决的核心问题。为此，我们使用ribokinase (2fv7 Chain: B)人类来源的酶作为模板，以创建BMI RBKS 蛋白的同源模型。我们的结果表明：猪RBKS 蛋白中的(RBKS, 17-323AA)区域存在一种αβα构成的三明治结构以及β-barrel，表明了这些区域在表达RBKS蛋白活性起着重要作用。第一个猪的RBKS模型为在设计研究时理解RBKS 如何与位置蛋白配体之间作用，以了解其各类功能特性将有可能提供有用的参考。 |
2楼2011-09-01 10:14:59
phu_grassman
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爱与雨下(金币+1): 感谢你的支持！ 2011-09-01 15:37:23
huojinlong8610(金币+10, 翻译EPI+1): 2011-09-01 16:17:31
sltmac(金币+35): 欢迎常来~~ 2011-09-07 07:48:00
爱与雨下(金币+1): 感谢你的支持！ 2011-09-01 15:37:23
huojinlong8610(金币+10, 翻译EPI+1): 2011-09-01 16:17:31
sltmac(金币+35): 欢迎常来~~ 2011-09-07 07:48:00
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The signal peptide prediction performed by SignalP 3.0 on the basis of a combination of several artificial neural networks and hidden Markov models revealed that BMI RBKS contained a potential signal peptide with 58.8% probability. However, （加when）we search（时态是不是应该一致） signal peptide using human and cattle RBKS protein sequences by SignalP 3.0, there is not a singal peptide found, the probability is low 1%（as low as 1%）. The reason about (The reason for the )difference and conflict of pig , human and cattle RBKS signal peptide need further study later(needs further study, later可删去). RBKS was discovered more than 50 years ago, and there has been much progress in understanding this important enzyme within the last decade. But the molecular mechanisms of which actors and how target it （这一句可否提供中文翻译）remain a central unresolved problem for biochemists and pharmacologists. For this reason, the homology modeling of the BMI RBKS protein was carried out using human ribokinase (2fv7 Chain: B) as the template. Our results indicate that the conserved domain (RBKS, 17-323AA) of pig RBKS protein mainly exists（exist 为不及物动词，是不是以这两种形式存在，可以在前面加in the form of） αβα sandwich domain and β-barrel. It suggests that these domains may play a pivotal role in RBKS activity. This first pig structural model of RBKS should prove useful reference in designing studies aimed at understanding how RBKS interacts with unknown protein partners for explaining its various functions. , |
6楼2011-09-01 13:55:01
