Originally posted by sunlblove at 2011-05-23 19:35:38:
怎么跑这么长时间啊 最下面的都跑出去了  
一般细菌16s都是1.5KB左右，你是用的通用引物么？

Originally posted by rainwander at 2011-05-23 19:46:49:
没说明用的哪对引物

Originally posted by bear0329 at 2011-05-23 20:34:51:
用的引物是27f/907r。

Originally posted by rainwander at 2011-05-23 20:37:45:
那就差不多对了，

送去测序看看，

还以为你用的是27F+11492R呢

Originally posted by bear0329 at 2011-05-23 20:42:57:
我是想做T-RFLP的，27f加了荧光标记，看了文献说细菌16SrDNA一般都1.5KB，我不敢确定，我也是刚接触分子，好多都不懂。谢谢你啊~
如果可以的话，就多P几管出来，然后回收纯化，再酶切送检了。