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legend0463

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在高等生物蛋白组学研究中发现,逆境条件下某蛋白A含量远远高于它在正常条件下的含量,但是在mRNA水平上检测表明,在逆境条件和正常条件下此基因的表达水平基本一致。请根据你所学的知识说明其可能的表达调控机制。
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Originally posted by legend0463 at 2011-03-09 10:26:42:
Just as you say, another reason is the effection of miRNA at translational level.  In this mode of action, miRNA usually targets the 3'-UTR of mRNA and inhibite the protein production.

in my study it is not the case you talked
there are some proteins which are involved in mRNA binding and target mRNA to ribosome, when these proteins are deleted, some specific mRNAs are still expressed but less mRNAs appear in the ribosome, therefore the protein synthesis is reduced
6楼2011-03-09 15:26:13
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legend0463(金币+1): Thanks! To be continued? 2011-03-08 22:14:49
看天(金币+2): 鼓励! 2011-03-09 10:54:05
one possibility is under stress conditions, the protein stability is much stronger than the normal conditions, means the half life is longer which might be due to post translationally modification
another possibility is -------

[ Last edited by wangleisdnu on 2011-3-8 at 21:39 ]
2楼2011-03-08 21:38:23
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chenjunustb

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legend0463(金币+1): 多谢讨论! 2011-03-09 10:21:42
看天(金币+2): 鼓励 2011-03-09 10:54:27
我觉得是在逆境的条件下,促进了该蛋白的翻译水平的提高,另外的可能是这种蛋白本身存在降解机制,但是由于逆境的出现,这种机制受到了抑制,导致该蛋白的积累。
3楼2011-03-08 22:46:00
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看天(金币+3): 强人 2011-03-09 10:55:30
引用回帖:
Originally posted by chenjunustb at 2011-03-08 22:46:00:
我觉得是在逆境的条件下,促进了该蛋白的翻译水平的提高,另外的可能是这种蛋白本身存在降解机制,但是由于逆境的出现,这种机制受到了抑制,导致该蛋白的积累。

you know the answer more than me
I know there are some genes which are not affected at mRNA level but the protein level is severely reduced, one reason is the specific mRNA can not be efficiently attached to the ribosome,
4楼2011-03-09 01:47:09
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