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To check quality, analyze 100 ng of your mRNA using a Bioanalyzer, or by
performing agarose gel electrophoresis using a 1% E-Gel® EX Gel (see page 45 for ordering information). Your mRNA will appear as a smear, with the greatest intensity in the range of 1 to 3 kb. Some rRNA bands may still be faintly visible. If you do not detect a smear, or if the majority of the smear is significantly lessthan 1 kb, you will need to repeat the RNA isolation. Be sure to follow the recommendations listed on the previous page to prevent RNase contamination.


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sai00fuji

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sai00fuji

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Originally posted by ·È˼×007 at 2011-02-25 22:51:39:
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To check quality, analyze 100 ng of your mRNA using a Bioanalyzer, or by
performing agarose gel electrophoresis using a 1% E-Gel® EX Gel (see page 45 for ordering information). Your mRNA will appear as a smear, with the greatest intensity in the range of 1 to 3 kb. Some rRNA bands may still be faintly visible. If you do not detect a smear, or if the majority of the smear is significantly lessthan 1 kb, you will need to repeat the RNA isolation. Be sure to follow the recommendations listed on the previous page to prevent RNase contamination.


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sai00fuji

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Originally posted by ·È˼×007 at 2011-02-26 19:49:38:
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sai00fuji(½ð±Ò+3): лл 2011-02-27 12:35:59
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hyphen007

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9Â¥: Originally posted by ·È˼×007 at 2011-02-26 20:48:44
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