24小时热门版块排行榜

返回列表

wncgliu86

金虫 (正式写手)

应助: 10 (幼儿园)
金币: 245.4
帖子: 397
在线: 148.2小时
虫号: 294671

[交流] 【求助】麻烦大家帮我分析一下这亲核取代反应

麻烦各位帮我分析一下影响这个反应的主要因素，胺试剂可以选择巯乙胺或者苄硫醇，这三种亲核试剂哪个更容易反应？谢谢，我再把实验操作给大家：

Thiolysis：100 ul of the sample were mixed with 100 ul of methanol acidified by concentrated HCl (3.3% v/v), and 200 ll of toluene-a-thiol (5% v/v in methanol) were added. The reaction mixture was placed in a sealed Pyrex glass test tube, heated at 40 C for 30 min and finally cooled with running water.

cysteamine ：The lyophilizate (1 mg) was dissolved in MeOH (1 mL). An aliquot (200 ixL) was added to the thiolysis mixture (200 ixL), which consisted of cysteamine hydrochloride (50 mg) and 37% hydrochloric acid (20 ixL) dissolved in MeOH (930 ixL).The mixture (400 ixL) was kept at 60 ~for 15 min, when the reaction was then quenched with 0.1% (v/v) aqueous TFA (1.2 mL).

phloroglucinol：sample were dissolved in 400 ll of freshly prepared MeOH containing 0.2 N HCl, 50 g/l phloroglucinol, and 10 g/l ascorbic acid and heated for 60 min at 50 C. Then, an equivalent volume of aqueous 200 mM sodium acetate was added to stop the reaction。

回复此楼