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bigboy123

铜虫 (小有名气)


[交流] 【求助/交流】帮忙看看这是什么东西(talin、paxillion),怎么都查不到呀

这是一朋友要用到的三个蛋白的单抗,查半天没搞清具体是什么?

FAK、talin、paxillion三个蛋白,要做细胞免疫荧光的实验,找三个不同种属的单抗和不同标记的二抗。FAK我知道,后两样怎么没听过呀。

[ Last edited by bigboy123 on 2010-12-12 at 09:50 ]
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bigboy123(金币+1):多谢,我也感觉是写错了,你的解释是非常合理的。 2010-12-12 17:52:44
http://en.wikipedia.org/wiki/Paxillin

Paxillin is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge[1] and should not be confused with the neurotoxin paxilline. The C-terminal region of paxillin contains four LIM domains which target paxillin to focal adhesions, presumably through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein-protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk

不知道你最后一个词是否打错了。
4楼2010-12-12 11:02:05
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hfliup98

木虫 (著名写手)


bigboy123(金币+1):多谢支持。 2010-12-12 17:53:28
talin 踝蛋白[膜下的一种细胞骨架蛋白,见于粘着斑]
2楼2010-12-12 10:12:33
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yanglove

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3楼2010-12-12 10:50:13
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